Probing Asymmetric Behavior of a Cell Cycle Regupatory Protein in Live Caulobacter using Single-Molecule Imaging

Asymmetric cell division is a fundamental event in developmental programs where two daughter cells are produced and exhibit distinct characteristics and fates. Remarkably, asymmetric cell division is also observed in the bacterium Caulobacter crescentus (∼3μm x 0.5μm). Caulobacter regulates cell cycle events through its master regulator CtrA, which is specifically degraded at one cell pole (stalked) during the swarmer-to-stalk (G1-to-S)...