Original paper
Unparalleled sample treatment throughput for proteomics workflows relying on ultrasonic energy
Abstract
We report on the new microplate horn ultrasonic device as a powerful tool to speed proteomics workflows with unparalleled throughput. 96 complex proteomes were digested at the same time in 4 min. Variables such as ultrasonication time, ultrasonication amplitude, and protein to enzyme ratio were optimized. The "classic" method relying on overnight protein digestion (12 h) and the sonoreactor-based method were also employed for comparative...
Paper Details
Title
Unparalleled sample treatment throughput for proteomics workflows relying on ultrasonic energy
Published Date
Feb 1, 2018
Journal
Volume
178
Pages
1067 - 1076
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Notes
History