Unparalleled sample treatment throughput for proteomics workflows relying on ultrasonic energy

We report on the new microplate horn ultrasonic device as a powerful tool to speed proteomics workflows with unparalleled throughput. 96 complex proteomes were digested at the same time in 4 min. Variables such as ultrasonication time, ultrasonication amplitude, and protein to enzyme ratio were optimized. The "classic" method relying on overnight protein digestion (12 h) and the sonoreactor-based method were also employed for comparative...