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Susana Jorge
Universidade Nova de Lisboa
7Publications
3H-index
34Citations
Publications 7
Newest
#1Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.José Luis Capelo-Martínez (NOVA: Universidade Nova de Lisboa)H-Index: 4
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Abstract A novel analytical approach is proposed to discriminate between solid biopsies of chromophobe renal cell carcinoma (chRCC) and renal oncocytoma (RO). The method comprises the following steps: (i) ultrasonic extraction of proteins from solid biopsies, (ii) protein depletion with acetonitrile, (iii) ultrasonic assisted in-solution digestion using magnetic nanoparticle with immobilized trypsin, (iv) C18 tip-based preconcentration of peptides, (v) sequential extraction of the peptides with ...
#1J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
#2Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.J.L. Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 3
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Abstract The adverse effects of air pollution have been long studied in the lung and respiratory systems, but the molecular changes that this causes at the central nervous system level have yet to be fully investigated and understood. To explore the evolution with time of protein expression levels in the brain of rats exposed to particulate matter of different sizes, we carried out two-dimensional gel electrophoresis followed by determination of dysregulated proteins through Coomassie blue stain...
#1Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
#2José Luis Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 30
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
view all 6 authors...
An effective three-step proteomics workflow is proposed to overcome the pitfalls caused by polymers present in optimum cutting temperature (OCT)-embedded tissue during its preparation for mass spectrometry analysis. First, the OCT-embedded tissue biopsies are cleaned using ethanol and water in a sequential series of ultrasonic washes in an ultrasound bath (35 kHz ultrasonic frequency, 100% ultrasonic amplitude, 2 min of ultrasonic duty time). Second, a fast ultrasonic-assisted extraction of prot...
#1Elisabete Oliveira (NOVA: Universidade Nova de Lisboa)H-Index: 19
#2Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
Last.Carlos Lodeiro (NOVA: Universidade Nova de Lisboa)H-Index: 32
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Abstract The synthesis and full characterization of a family of sustainable luminescent nanoparticles, CdTeQDs@MNs, made of quantum dots coated by modified mesoporous silica nanoparticles is reported. The presence of the luminescent QDs allows the imaging of the nanoparticles during cell internalization, as well as the visualization of selective release of doxorubicin inside the cell (via pH stimuli). The modified mesoporous silica nanoparticles lead to pore size improvement allowing better enca...
#1Hugo López-Fernández (University of Vigo)H-Index: 8
#2J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
Last.José Luis Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 30
view all 8 authors...
Abstract Background and objective 2D-gel electrophoresis is widely used in combination with MALDI-TOF mass spectrometry in order to analyze the proteome of biological samples. For instance, it can be used to discover proteins that are differentially expressed between two groups (e.g. two disease conditions, case vs. control, etc.) thus obtaining a set of potential biomarkers. This procedure requires a great deal of data processing in order to prepare data for analysis or to merge and integrate d...
#1Susana JorgeH-Index: 3
#2J.E. AraújoH-Index: 7
Last.José Luis CapeloH-Index: 30
view all 7 authors...
Abstract We report on the new microplate horn ultrasonic device as a powerful tool to speed proteomics workflows with unparalleled throughput. 96 complex proteomes were digested at the same time in 4 min. Variables such as ultrasonication time, ultrasonication amplitude, and protein to enzyme ratio were optimized. The "classic" method relying on overnight protein digestion (12 h) and the sonoreactor-based method were also employed for comparative purposes. We found the protein digestion efficien...
#1J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
#2Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.José Luis Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 30
view all 7 authors...
Abstract Protein depletion with acetonitrile and protein equalization with dithiothreitol have been assessed with success as proteomics tools for getting insight into the peritoneal dialysate effluent proteome. The methods proposed are cost-effective, fast and easy of handling, and they match the criteria of analytical minimalism: low sample volume and low reagent consumption. Using two-dimensional gel electrophoresis and peptide mass fingerprinting, a total of 72 unique proteins were identified...
#1J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
#2Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
view all 10 authors...
Protein equalization with dithiothreitol, protein depletion with acetonitrile and the entire proteome were assessed in conjunction with matrix assisted laser desorption ionization time of flight mass spectrometry-based profiling for a fast and effective classification of patients with renal insufficiency. Two case groups were recruited as proof of concept, patients with chronic glomerulonephritis and diabetic nephropathy. Two key tools were used to develop this approach: protein concentration wi...
#1J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
#2Tiago Santos (UTAD: University of Trás-os-Montes and Alto Douro)H-Index: 6
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
view all 10 authors...
The aim of this study was to differentiate patients with glomerulonephritis and diabetic nephropathy using (i) peritoneal dialysate effluent, (ii) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and (iii) bioinformatics tools. Profiles of peritoneal dialysate effluent were obtained using (a) sample preparation consisting of protein concentration through centrifugal concentrators and chemical-assisted protein depletion using DL-dithiothreitol, and (b) M...
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