Susana Jorge
Universidade Nova de Lisboa
7Publications
3H-index
33Citations
Publications 7
Newest
Published on Feb 1, 2019in Dyes and Pigments 3.77
Elisabete Oliveira19
Estimated H-index: 19
(Universidade Nova de Lisboa),
Hugo M. Santos17
Estimated H-index: 17
(Universidade Nova de Lisboa)
+ 6 AuthorsCarlos Lodeiro32
Estimated H-index: 32
(Universidade Nova de Lisboa)
Abstract The synthesis and full characterization of a family of sustainable luminescent nanoparticles, CdTeQDs@MNs, made of quantum dots coated by modified mesoporous silica nanoparticles is reported. The presence of the luminescent QDs allows the imaging of the nanoparticles during cell internalization, as well as the visualization of selective release of doxorubicin inside the cell (via pH stimuli). The modified mesoporous silica nanoparticles lead to pore size improvement allowing better enca...
2 Citations Source Cite
Published on Mar 1, 2018in Computer Methods and Programs in Biomedicine 2.67
Hugo López-Fernández7
Estimated H-index: 7
(University of Vigo),
J.E. Araújo7
Estimated H-index: 7
(Universidade Nova de Lisboa)
+ 5 AuthorsJosé Luis Capelo29
Estimated H-index: 29
(Universidade Nova de Lisboa)
Abstract Background and objective 2D-gel electrophoresis is widely used in combination with MALDI-TOF mass spectrometry in order to analyze the proteome of biological samples. For instance, it can be used to discover proteins that are differentially expressed between two groups (e.g. two disease conditions, case vs. control, etc.) thus obtaining a set of potential biomarkers. This procedure requires a great deal of data processing in order to prepare data for analysis or to merge and integrate d...
1 Citations Source Cite
Published on Feb 1, 2018in Talanta 4.24
Susana Jorge3
Estimated H-index: 3
,
J.E. Araújo7
Estimated H-index: 7
+ 4 AuthorsJosé Luis Capelo29
Estimated H-index: 29
Abstract We report on the new microplate horn ultrasonic device as a powerful tool to speed proteomics workflows with unparalleled throughput. 96 complex proteomes were digested at the same time in 4 min. Variables such as ultrasonication time, ultrasonication amplitude, and protein to enzyme ratio were optimized. The "classic" method relying on overnight protein digestion (12 h) and the sonoreactor-based method were also employed for comparative purposes. We found the protein digestion efficien...
2 Citations Source Cite
José Luis Capelo29
Estimated H-index: 29
,
Hugo M. Santos17
Estimated H-index: 17
+ 1 AuthorsSusana Jorge3
Estimated H-index: 3
Source Cite
Published on May 1, 2016in Talanta 4.24
J.E. Araújo7
Estimated H-index: 7
(Universidade Nova de Lisboa),
Susana Jorge3
Estimated H-index: 3
(Universidade Nova de Lisboa)
+ 7 AuthorsHugo M. Santos17
Estimated H-index: 17
(Universidade Nova de Lisboa)
Protein equalization with dithiothreitol, protein depletion with acetonitrile and the entire proteome were assessed in conjunction with matrix assisted laser desorption ionization time of flight mass spectrometry-based profiling for a fast and effective classification of patients with renal insufficiency. Two case groups were recruited as proof of concept, patients with chronic glomerulonephritis and diabetic nephropathy. Two key tools were used to develop this approach: protein concentration wi...
7 Citations Source Cite
Published on Aug 1, 2016in Journal of Proteomics 3.72
J.E. Araújo7
Estimated H-index: 7
(Universidade Nova de Lisboa),
Susana Jorge3
Estimated H-index: 3
(Universidade Nova de Lisboa)
+ 4 AuthorsJosé Luis Capelo29
Estimated H-index: 29
(Universidade Nova de Lisboa)
Abstract Protein depletion with acetonitrile and protein equalization with dithiothreitol have been assessed with success as proteomics tools for getting insight into the peritoneal dialysate effluent proteome. The methods proposed are cost-effective, fast and easy of handling, and they match the criteria of analytical minimalism: low sample volume and low reagent consumption. Using two-dimensional gel electrophoresis and peptide mass fingerprinting, a total of 72 unique proteins were identified...
12 Citations Source Cite
Published on Jan 1, 2015in Analytical Methods 2.07
J.E. Araújo7
Estimated H-index: 7
(Universidade Nova de Lisboa),
Tiago Santos6
Estimated H-index: 6
(University of Trás-os-Montes and Alto Douro)
+ 7 AuthorsHugo M. Santos17
Estimated H-index: 17
(Universidade Nova de Lisboa)
The aim of this study was to differentiate patients with glomerulonephritis and diabetic nephropathy using (i) peritoneal dialysate effluent, (ii) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and (iii) bioinformatics tools. Profiles of peritoneal dialysate effluent were obtained using (a) sample preparation consisting of protein concentration through centrifugal concentrators and chemical-assisted protein depletion using DL-dithiothreitol, and (b) M...
9 Citations Source Cite
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