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José Luis Capelo
Universidade Nova de Lisboa
220Publications
30H-index
3,023Citations
Publications 220
Newest
#1Gonçalo A. Marcelo (NSU: Nova Southeastern University)
#2Carlos Lodeiro (NSU: Nova Southeastern University)
Last.Elisabete Oliveira (NSU: Nova Southeastern University)
view all 5 authors...
Abstract Multifunctional nanoparticles have emerged as an outstanding candidate for a new generation of biomedical applications, mainly due to their remarkable properties and biocompatibility. Individual reports on multi-metal, semiconducting and superparamagnetic nanoparticles (SPIONs), elucidating on each's unique intrinsic properties, have demonstrated that the biological application of such materials is highly dependent of their size, shape, surface nature and core nature. However, reviews c...
#1Cristina dos Santos Mesquita (University of Minho)
#2Pedro Soares-Castro (University of Minho)H-Index: 4
Last.Pedro M. Santos (University of Minho)H-Index: 17
view all 6 authors...
Abstract In this work, a genotype-phenotype survey of a highly diversified Pseudomonas aeruginosa collection was conducted, aiming to detail pathogen-associated scenarios that clinicians face nowadays. Genetic relation based on RAPD-PCR of 705 isolates, retrieved from 424 patients and several clinical contexts, reported an almost isolate-specific molecular-pattern. Pneumonia-associated isolates HB13 and HB15, clustered in the same RAPD-PCR group, were selected to evaluate the genomic background ...
#1J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
#2Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.J.L. Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 3
view all 10 authors...
Abstract The adverse effects of air pollution have been long studied in the lung and respiratory systems, but the molecular changes that this causes at the central nervous system level have yet to be fully investigated and understood. To explore the evolution with time of protein expression levels in the brain of rats exposed to particulate matter of different sizes, we carried out two-dimensional gel electrophoresis followed by determination of dysregulated proteins through Coomassie blue stain...
#1Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
#2José Luis Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 30
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
view all 6 authors...
An effective three-step proteomics workflow is proposed to overcome the pitfalls caused by polymers present in optimum cutting temperature (OCT)-embedded tissue during its preparation for mass spectrometry analysis. First, the OCT-embedded tissue biopsies are cleaned using ethanol and water in a sequential series of ultrasonic washes in an ultrasound bath (35 kHz ultrasonic frequency, 100% ultrasonic amplitude, 2 min of ultrasonic duty time). Second, a fast ultrasonic-assisted extraction of prot...
Iron is the most abundant element by mass in the Earth. Despite being an inexpensive material used in a wide range of applications, its role as reducing agent for the synthesis of plasmonic nanoparticles has not been explored in detail. In the present work, we have studied the use of iron(II) sulfate as a green reductant for the synthesis of gold nanoparticles (AuNPs) in the absence or presence of different molecules. First, we demonstrate that iron(II), in the absence of any additive or capping...
The application of novel methodologies to the synthesis of nanomaterials is still a challenge in many different technological and scientific fields. New efficient and reproducible synthetic methodologies, that produce fewer residues and reduce the cost of raw materials must be developed. In the present work, we have explored the attractive possibility to apply the cheap iron (II) sulphate salt in the reduction process of the K2PtCl4 to produce colloids suspensions. The synthesis took places in w...
#1Gonçalo Martins (NOVA: Universidade Nova de Lisboa)
#2Javier Fernández-Lodeiro (NOVA: Universidade Nova de Lisboa)H-Index: 10
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
view all 6 authors...
Abstract The ultimate high-throughput, high robustness and easy-to-handling sample treatment method for label-free shotgun proteomics is presented in this work. It is based on joining the effectiveness of immobilized trypsin at the nanoscale level with the latest technology to deliver ultrasonic energy. The new method can be used to reduce sample preparation time comprising the steps of reduction, alkylation and digestion time to just 15 min without compromising shotgun label-free protein quanti...
#1Elisabete Oliveira (NOVA: Universidade Nova de Lisboa)H-Index: 19
#2Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
Last.Carlos Lodeiro (NOVA: Universidade Nova de Lisboa)H-Index: 32
view all 9 authors...
Abstract The synthesis and full characterization of a family of sustainable luminescent nanoparticles, CdTeQDs@MNs, made of quantum dots coated by modified mesoporous silica nanoparticles is reported. The presence of the luminescent QDs allows the imaging of the nanoparticles during cell internalization, as well as the visualization of selective release of doxorubicin inside the cell (via pH stimuli). The modified mesoporous silica nanoparticles lead to pore size improvement allowing better enca...
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