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Development of membrane ion channels during neural differentiation from human embryonic stem cells

Published on Sep 1, 2017in Biochemical and Biophysical Research Communications 2.71
· DOI :10.1016/j.bbrc.2017.07.068
Sara Mirsadeghi1
Estimated H-index: 1
(Royan Institute),
Ebrahim Shahbazi7
Estimated H-index: 7
(Royan Institute)
+ 4 AuthorsSahar Kiani14
Estimated H-index: 14
(Royan Institute)
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Abstract
Abstract Objective For human embryonic stem cells (hESCs) to differentiate into neurons, enormous changes has to occur leading to trigger action potential and neurotransmitter release. We attempt to determine the changes in expression of voltage gated channels (VGCs) and their electrophysiological properties during neural differentiation. Materials and methods The relative expressions of α-subunit of voltage gated potassium, sodium and calcium channels were characterized by qRT-PCR technique. Patch clamp recording was performed to characterize the electrophysiological properties of hESCs during their differentiation into neuron-like cells. Results Relative expression of α-subunit of channels changed significantly. 4-AP and TEA sensitive outward currents were observed in all stages, although TEA sensitive currents were recorded once in rosette structure. Nifedipine and QX314 sensitive inward currents were recorded only in neuron-like cells. Conclusion K + currents were recorded in hESCs and rosette structure cells. Inward currents, sensitive to Nifedipine and QX314, were recorded in neuron-like cells.
  • References (21)
  • Citations (2)
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References21
Newest
Published on Mar 1, 2016in Cell Calcium 3.93
Oksana Forostyak7
Estimated H-index: 7
(Charles University in Prague),
ForostyakSerhiy12
Estimated H-index: 12
(Charles University in Prague)
+ 3 AuthorsGovindan Dayanithi35
Estimated H-index: 35
(French Institute of Health and Medical Research)
Abstract Stem cells (SCs) of different origins have brought hope as potential tools for the treatment of neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, and Amyotrophic Lateral Sclerosis. Calcium signalling plays a key role in SC differentiation and proliferation, and dysregulation of Ca 2+ homeostasis may instigate pathological scenarios. Currently, the role of ion channels and receptors in SCs is not fully understood. In the recent years, we found that (i) the pre-...
Published on Mar 1, 2016in Regenerative Medicine 2.38
Ankshita Prasad3
Estimated H-index: 3
(NUS: National University of Singapore),
Janani Manivannan2
Estimated H-index: 2
(NUS: National University of Singapore)
+ 3 AuthorsAngelo Homayoun All17
Estimated H-index: 17
Rapid progress in the field of stem cell therapy and cellular reprogramming provides convincing evidence of its feasibility in treating a wide range of pathologies through autologous cell replacement therapy. This review article describes in detail on three widely used approaches of somatic cell reprogramming: induced pluripotent stem cells, direct conversion and direct reprogramming, in the context of demyelination in the CNS. The potential limitations of each reprogramming technique are review...
Published on Oct 1, 2012in Journal of Biomolecular Screening 2.30
Alison Haythornthwaite3
Estimated H-index: 3
,
Sonja Stoelzle8
Estimated H-index: 8
+ 5 AuthorsNiels Fertig22
Estimated H-index: 22
Neurons derived from human-induced pluripotent stem cells were characterized using manual and automated patch-clamp recordings. These cells expressed voltage-gated Na + (Na v ), Ca 2+ (Ca v ), and K + (K v ) channels as expected from excitable cells. The Na v current was TTX sensitive, IC 50 = 12 ± 6 nM (n = 5). About 50% of the Ca v current was blocked by 10 µM of the L-type channel blocker nifedipine. Two populations of the K v channel were present in different proportions: an inactivating (A-...
Published on Dec 1, 2011in Tissue Engineering Part A
Ebrahim Shahbazi7
Estimated H-index: 7
,
Sahar Kiani14
Estimated H-index: 14
+ 1 AuthorsHossein Baharvand42
Estimated H-index: 42
In this study, the impact of randomly oriented electrospun polyamide nanofibrous architecture on neurogenic differentiation of human embryonic stem cells (hESCs) compared with the lack of nanofibrous features in vitro in a neural-inducing condition was examined. Flow cytometry analysis of hESC-derived neural ectoderm (NE) showed nanofibrous surfaces capable of supporting NE by expression of higher percentages of related markers NESTIN, SOX1, and PAX6 in addition to significantly greater total ce...
Published on Sep 1, 2011in Neuroscience 3.24
Amber Young5
Estimated H-index: 5
,
Dave W. Machacek5
Estimated H-index: 5
+ 5 AuthorsSteven L. Stice35
Estimated H-index: 35
Abstract Human neural progenitor cells differentiated from human embryonic stem cells offer a potential cell source for studying neurodegenerative diseases and for drug screening assays. Previously, we demonstrated that human neural progenitors could be maintained in a proliferative state with the addition of leukemia inhibitory factor and basic fibroblast growth factor. Here we demonstrate that 96 h after removal of basic fibroblast growth factor the neural progenitor cell culture was significa...
Published on Jan 1, 2011in World Journal of Stem Cells
Gui-Rong Li1
Estimated H-index: 1
(HKU: University of Hong Kong),
Xiu-Ling Deng1
Estimated H-index: 1
Bioelectrical signals generated by ion channels play crucial roles in excitation genesis and impulse conduction in excitable cells as well as in cell proliferation, migration and apoptosis in proliferative cells. Recent studies have demonstrated that multiple ion channels are heterogeneously present in different stem cells; however, patterns and phenotypes of ion channels are species- and/ or origin-dependent. This editorial review focuses on the recent findings related to the expression of func...
Published on Apr 1, 2010in Cell Transplantation 3.48
Nataliya Kozubenko3
Estimated H-index: 3
(Charles University in Prague),
Karolina Turnovcova10
Estimated H-index: 10
(Charles University in Prague)
+ 7 AuthorsEva Syková10
Estimated H-index: 10
(Charles University in Prague)
Abstract During the last decade, much progress has been made in developing protocols for the differentiation of human embryonic stem cells (hESCs) into a neural phenotype. The appropriate agent for cell therapy is neural precursors (NPs). Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating NPs from the CCTL14 line of hESCs. These NPs could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and elect...
Published on Mar 1, 2010in American Journal of Physiology-cell Physiology 3.55
Peng Jiang13
Estimated H-index: 13
(UC Davis: University of California, Davis),
Stephanie N. Rushing6
Estimated H-index: 6
(UC Davis: University of California, Davis)
+ 5 AuthorsRonald A. Li40
Estimated H-index: 40
(UC Davis: University of California, Davis)
Human embryonic stem cells (hESCs) can self-renew while maintaining their pluripotency. Direct reprogramming of adult somatic cells to induced pluripotent stem cells (iPSCs) has been reported. Although hESCs and human iPSCs have been shown to share a number of similarities, such basic properties as the electrophysiology of iPSCs have not been explored. Previously, we reported that several specialized ion channels are functionally expressed in hESCs. Using transcriptomic analyses as a guide, we o...
Published on Nov 1, 2009in Journal of Neuroscience Methods 2.79
Mathilda Zetterström Axell5
Estimated H-index: 5
(University of Gothenburg),
Suzana Zlateva1
Estimated H-index: 1
(University of Gothenburg),
Maurice A. Curtis28
Estimated H-index: 28
(University of Auckland)
Neuronal loss is a common feature of many neurological disorders, including stroke, Parkinson's disease, Alzheimer's disease and traumatic brain injury. Human embryonic stem cell (hESC)-derived neural progenitors (NPs) may provide new ways of treatment for several diseases and injuries in the brain, as well as enhance our understanding of early human development. Here we report a method for rapid generation of proliferating NPs from feeder free cultures of undifferentiated hESCs. In this rapid a...
Published on Oct 6, 2009in PLOS ONE 2.78
Gui-Rong Li27
Estimated H-index: 27
(Li Ka Shing Faculty of Medicine, University of Hong Kong),
Hai-Ying Sun19
Estimated H-index: 19
(Li Ka Shing Faculty of Medicine, University of Hong Kong)
+ 3 AuthorsCp Lau29
Estimated H-index: 29
(Li Ka Shing Faculty of Medicine, University of Hong Kong)
Background: Although fibroblast-to-myocyte electrical coupling is experimentally suggested, electrophysiology of cardiac fibroblasts is not as well established as contractile cardiac myocytes. The present study was therefore designed to characterize ion channels in cultured human cardiac fibroblasts. Methods and Findings: A whole-cell patch voltage clamp technique and RT-PCR were employed to determine ion channels expression and their molecular identities. We found that multiple ion channels wer...
Cited By2
Newest
Published on Jun 1, 2019in Progress in Biophysics & Molecular Biology 2.70
Abstract The largest biomass of channel proteins is located in unicellular organisms and bacteria that have no organs. However, orchestrated bidirectional ionic currents across the cell membrane via the channels are important for the functioning of organs of organisms, and equally concern both fauna or flora. Several ion channels are activated in the course of action potentials. One of the hallmarks of voltage-dependent channels is a ‘tail current’ − deactivation as observed after prior and suff...
Published on Nov 1, 2018in Science China-life Sciences 3.58
Man Kit Tse1
Estimated H-index: 1
(HKUST: Hong Kong University of Science and Technology),
Ting Shing Hung1
Estimated H-index: 1
(HKUST: Hong Kong University of Science and Technology)
+ 6 AuthorsSarah E. Webb15
Estimated H-index: 15
(HKUST: Hong Kong University of Science and Technology)
The development of the CNS in vertebrate embryos involves the generation of different sub-types of neurons and glia in a complex but highly-ordered spatio-temporal manner. Zebrafish are commonly used for exploring the development, plasticity and regeneration of the CNS, and the recent development of reliable protocols for isolating and culturing neural stem/progenitor cells (NSCs/NPCs) from the brain of adult fish now enables the exploration of mechanisms underlying the induction/specification/d...