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CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein

Published on Jun 1, 2017in Molecular Genetics and Genomics2.88
· DOI :10.1007/s00438-017-1299-z
Lichun Tang1
Estimated H-index: 1
(Protein Sciences),
Yanting Zeng1
Estimated H-index: 1
(Guangzhou Medical University)
+ 8 AuthorsJianqiao Liu6
Estimated H-index: 6
(Guangzhou Medical University)
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Abstract
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, we demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, our results also reveal limitations of this correction procedure and highlight the need for further research.
  • References (27)
  • Citations (91)
References27
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#1Meilin ZhuH-Index: 1
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#1Masakazu Hashimoto (Osaka University)H-Index: 7
#2Yukiko Yamashita (University of Tokushima)H-Index: 2
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#2Thomas J. Cradick (Georgia Institute of Technology)H-Index: 18
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#1Sojung Kim (SNU: Seoul National University)H-Index: 11
#2Daesik Kim (SNU: Seoul National University)H-Index: 14
Last.Jin-Soo Kim (SNU: Seoul National University)H-Index: 53
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