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Generation of transgenic marmosets expressing genetically encoded calcium indicators

Published on Dec 1, 2016in Scientific Reports4.01
· DOI :10.1038/srep34931
Jung Eun Park1
Estimated H-index: 1
,
Xian Feng Zhang2
Estimated H-index: 2
+ 3 AuthorsAfonso C. Silva35
Estimated H-index: 35
Abstract
Chronic monitoring of neuronal activity in the living brain with optical imaging techniques became feasible owing to the continued development of genetically encoded calcium indicators (GECIs). Here we report for the first time the successful generation of transgenic marmosets (Callithrix jacchus), an important nonhuman primate model in neurophysiological research, which were engineered to express the green fluorescent protein (GFP)-based family of GECIs, GCaMP, under control of either the CMV or the hSyn promoter. High titer lentiviral vectors were produced, and injected into embryos collected from donor females. The infected embryos were then transferred to recipient females. Eight transgenic animals were born and shown to have stable and functional GCaMP expression in several different tissues. Germline transmission of the transgene was confirmed in embryos generated from two of the founder transgenic marmosets that reached sexual maturity. These embryos were implanted into six recipient females, three of which became pregnant and are in advanced stages of gestation. We believe these transgenic marmosets will be invaluable non-human primate models in neuroscience, allowing chronic in vivo monitoring of neural activity with functional confocal and multi-photon optical microscopy imaging of intracellular calcium dynamics.
  • References (75)
  • Citations (24)
References75
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#1Cory T. Miller (UCSD: University of California, San Diego)H-Index: 19
#2Winrich A. Freiwald (Rockefeller University)H-Index: 18
Last.Xiaoqin Wang (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 44
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#2Bo Zheng (Nanjing Medical University)H-Index: 8
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#1Kornélia Szebényi (MTA: Hungarian Academy of Sciences)H-Index: 9
#2András Füredi (MTA: Hungarian Academy of Sciences)H-Index: 6
Last.Gergely Szakács (MTA: Hungarian Academy of Sciences)H-Index: 33
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#1Sean Moran (Emory University)H-Index: 7
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#1Leila Ghanbari (UMN: University of Minnesota)H-Index: 1
#2Russell E. Carter (UMN: University of Minnesota)H-Index: 4
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#1Wakako Kumita (Central Institute for Experimental Animals)H-Index: 2
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#2Melina E. Hale (U of C: University of Chicago)H-Index: 25
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