Genetic evidence that InhA of Mycobacterium smegmatis is a target for triclosan.

Published on Mar 1, 1999in Antimicrobial Agents and Chemotherapy4.715
· DOI :10.1128/AAC.43.3.711
Laura M. McMurry24
Estimated H-index: 24
Patrick F. McDermott52
Estimated H-index: 52
Stuart B. Levy78
Estimated H-index: 78
(Tufts University)
Three Mycobacterium smegmatis mutants selected for resistance to triclosan each had a different mutation in InhA, an enoyl reductase involved in fatty acid synthesis. Two expressed some isoniazid resistance. A mutation originally selected on isoniazid also mediated triclosan resistance, as did the wild-type inhA gene on a multicopy plasmid. Replacement of the mutant chromosomal inhA genes with wild-type inhA eliminated resistance. These results suggest that M. smegmatis InhA, like its Escherichia coli homolog FabI, is a target for triclosan.
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Preface The Enteric Bacterial Cell and the Age of Bacteria Variations on a Theme by Escherichia Part I: Molecular Architecture and Assembly of Cell Parts (11 chapters) Part II: Metabolism and General Physiology (58 chapters) Part III: Utilization of Energy for Cell Activities (7 chapters) Part IV: Regulation of Gene Expression (19 chapters) Part V: Growth of Cells and Cultures (12 chapters) Part VI: Genome, Genetics and Evolution (40 chapters) Part VII: Molecular Pathogenesis (7 chapters)
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