Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
Abstract
Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to specific genomic loci by a 20 nt guide sequence, which can tolerate certain mismatches to the DNA target and thereby promote undesired off-target mutagenesis. Here, we describe an approach that combines a Cas9 nickase mutant with paired guide RNAs to introduce targeted...
Paper Details
Title
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
Published Date
Sep 1, 2013
Journal
Volume
154
Issue
6
Pages
1380 - 1389
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