Match!
Patrick Hsu
Salk Institute for Biological Studies
53Publications
15H-index
17.6kCitations
Publications 53
Newest
#1Chidiebere Uzodinma Awah (NU: Northwestern University)H-Index: 1
#2Li Chen (NU: Northwestern University)H-Index: 1
Last.Cheol Hong Park (NU: Northwestern University)H-Index: 4
view all 23 authors...
Topoisomerase II poisons are one of the most common class of chemotherapeutics used in cancer. We show that glioblastoma (GBM), the most malignant of all primary brain tumors in adults is responsive to TOP2 poisons. To identify genes that confer susceptibility to this drug in gliomas, we performed a genome-scale CRISPR knockout screen with etoposide. Genes involved in protein synthesis and DNA damage were implicated in etoposide susceptibility. To define potential biomarkers for TOP2 poisons, CR...
#1Josh Tycko (Stanford University)H-Index: 3
#2Luis A. BarreraH-Index: 14
Last.Patrick Hsu (Salk Institute for Biological Studies)H-Index: 15
view all 10 authors...
The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’, instead of the correct ‘Present address: Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’. It also incorrectly listed an affiliation of this author as ‘Present address: Arrakis Therapeutics, 35 Gatehouse Dr., Waltham, MA, 02451, USA’.The original HTML version incorrectly list...
#1Josh Tycko (Stanford University)H-Index: 3
#2Luis A. BarreraH-Index: 14
Last.Patrick Hsu (Salk Institute for Biological Studies)H-Index: 15
view all 10 authors...
Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing variation in human cells across a large repertoire of 88,692 single guide RNAs (sgRNAs) paired with matched or mismatched target sites in a synthetic cassette. We incorporate randomized barcodes that enable whitelisting of correctly synthesized molecules ...
#1Chidiebere Uzodinma Awah (NU: Northwestern University)H-Index: 1
#2Edgar Gonzalez-Buendia (NU: Northwestern University)
Last.Jan Winter (DKFZ: German Cancer Research Center)H-Index: 5
view all 18 authors...
#1Cheng Zhang (Salk Institute for Biological Studies)H-Index: 2
#2Silvana Konermann (Salk Institute for Biological Studies)H-Index: 11
Last.Dmitry Lyumkis (Salk Institute for Biological Studies)H-Index: 17
view all 10 authors...
Summary CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy str...
#1Cheng Zhang (Salk Institute for Biological Studies)H-Index: 2
#2Silvana Konermann (Salk Institute for Biological Studies)H-Index: 11
Last.Dmitry Lyumkis (Salk Institute for Biological Studies)H-Index: 17
view all 9 authors...
CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function, we resolved cryo-electron microscopy structures of Cas13d-guide RNA binary complex and Cas13d-...
#1Silvana Konermann (Salk Institute for Biological Studies)H-Index: 11
#2Peter Lotfy (Salk Institute for Biological Studies)H-Index: 2
Last.Patrick Hsu (Salk Institute for Biological Studies)H-Index: 15
view all 6 authors...
Summary Class 2 CRISPR-Cas systems endow microbes with diverse mechanisms for adaptive immunity. Here, we analyzed prokaryotic genome and metagenome sequences to identify an uncharacterized family of RNA-guided, RNA-targeting CRISPR systems that we classify as type VI-D. Biochemical characterization and protein engineering of seven distinct orthologs generated a ribonuclease effector derived from Ruminococcus flavefaciens XPD3002 (CasRx) with robust activity in human cells. CasRx-mediated knockd...
#1Josh TyckoH-Index: 3
#2Vic E. MyerH-Index: 19
Last.Patrick Hsu (Salk Institute for Biological Studies)H-Index: 15
view all 3 authors...
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing, ...
#1Silvana KonermannH-Index: 11
#2Mark D. BrighamH-Index: 12
Last.Feng Zhang (Broad Institute)H-Index: 104
view all 10 authors...
Here the customizable TALE DNA-binding domain was integrated with the light-sensitive cryptochrome 2 protein and its interacting partner (CIB1) from Arabidopsis thaliana, thereby creating an optogenetic two-hybrid system called light-inducible transcriptional effectors (LITEs); the LITE system establishes a novel mode of optogenetic control of endogenous transcription and epigenetic states.
1234