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Patrick Hsu
Salk Institute for Biological Studies
53Publications
15H-index
17.6kCitations
Publications 53
Newest
Published on Apr 25, 2019in bioRxiv
Chidiebere Uzodinma Awah1
Estimated H-index: 1
(Northwestern University),
Li Chen1
Estimated H-index: 1
(Northwestern University)
+ 20 AuthorsDaniel Zhang (Northwestern University)
Topoisomerase II poisons are one of the most common class of chemotherapeutics used in cancer. We show that glioblastoma (GBM), the most malignant of all primary brain tumors in adults is responsive to TOP2 poisons. To identify genes that confer susceptibility to this drug in gliomas, we performed a genome-scale CRISPR knockout screen with etoposide. Genes involved in protein synthesis and DNA damage were implicated in etoposide susceptibility. To define potential biomarkers for TOP2 poisons, CR...
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Published on Dec 1, 2018in Nature Communications 12.35
Josh Tycko3
Estimated H-index: 3
(Stanford University),
Luis A. Barrera14
Estimated H-index: 14
+ 7 AuthorsPatrick Hsu15
Estimated H-index: 15
(Salk Institute for Biological Studies)
The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’, instead of the correct ‘Present address: Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’. It also incorrectly listed an affiliation of this author as ‘Present address: Arrakis Therapeutics, 35 Gatehouse Dr., Waltham, MA, 02451, USA’.The original HTML version incorrectly list...
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Published on Dec 1, 2018in Nature Communications 12.35
Josh Tycko3
Estimated H-index: 3
(Stanford University),
Luis A. Barrera14
Estimated H-index: 14
+ 7 AuthorsPatrick Hsu15
Estimated H-index: 15
(Salk Institute for Biological Studies)
Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing variation in human cells across a large repertoire of 88,692 single guide RNAs (sgRNAs) paired with matched or mismatched target sites in a synthetic cassette. We incorporate randomized barcodes that enable whitelisting of correctly synthesized molecules ...
2 Citations Source Cite
Published on Nov 5, 2018in Neuro-oncology 9.38
Chidiebere Uzodinma Awah1
Estimated H-index: 1
(Northwestern University),
Edgar Gonzalez-Buendia (Northwestern University)+ 15 AuthorsJan Winter5
Estimated H-index: 5
(German Cancer Research Center)
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Published on Oct 3, 2018in Cell 31.40
Cheng Zhang2
Estimated H-index: 2
(Salk Institute for Biological Studies),
Silvana Konermann11
Estimated H-index: 11
(Salk Institute for Biological Studies)
+ 7 AuthorsDmitry Lyumkis17
Estimated H-index: 17
(Salk Institute for Biological Studies)
Summary CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy str...
9 Citations Source Cite
Published on Apr 1, 2018in Cell 31.40
Silvana Konermann11
Estimated H-index: 11
(Salk Institute for Biological Studies),
Peter Lotfy2
Estimated H-index: 2
(Salk Institute for Biological Studies)
+ 3 AuthorsPatrick Hsu15
Estimated H-index: 15
(Salk Institute for Biological Studies)
Summary Class 2 CRISPR-Cas systems endow microbes with diverse mechanisms for adaptive immunity. Here, we analyzed prokaryotic genome and metagenome sequences to identify an uncharacterized family of RNA-guided, RNA-targeting CRISPR systems that we classify as type VI-D. Biochemical characterization and protein engineering of seven distinct orthologs generated a ribonuclease effector derived from Ruminococcus flavefaciens XPD3002 (CasRx) with robust activity in human cells. CasRx-mediated knockd...
49 Citations Source Cite
Published on Sep 29, 2017
Patrick Hsu15
Estimated H-index: 15
,
Morgan L. Maeder27
Estimated H-index: 27
+ 2 AuthorsNicholas C. Huston1
Estimated H-index: 1
CRISPR/CAS-related compositions and methods for treatment of DMD, BMD, or DCM type 3B are described.
Published on Aug 1, 2016in Molecular Cell 14.25
Josh Tycko3
Estimated H-index: 3
,
Vic E. Myer19
Estimated H-index: 19
,
Patrick Hsu15
Estimated H-index: 15
(Salk Institute for Biological Studies)
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing, ...
85 Citations Source Cite
Published on Jun 2, 2016in Nature 41.58
Silvana Konermann11
Estimated H-index: 11
,
Mark D. Brigham12
Estimated H-index: 12
+ 7 AuthorsFeng Zhang104
Estimated H-index: 104
(Broad Institute)
Here the customizable TALE DNA-binding domain was integrated with the light-sensitive cryptochrome 2 protein and its interacting partner (CIB1) from Arabidopsis thaliana, thereby creating an optogenetic two-hybrid system called light-inducible transcriptional effectors (LITEs); the LITE system establishes a novel mode of optogenetic control of endogenous transcription and epigenetic states.
550 Citations Source Cite
Published on May 22, 2016
Silvana Konermann11
Estimated H-index: 11
,
Mark D. Brigham12
Estimated H-index: 12
+ 9 AuthorsOsamu Nureki53
Estimated H-index: 53
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