Original paper
Development of a reverse-genetics system for Avian pneumovirus demonstrates that the small hydrophobic (SH) and attachment (G) genes are not essential for virus viability
Abstract
Avian pneumovirus (APV) is a member of the genus Metapneumovirus of the subfamily Pneumovirinae . This study describes the development of a reverse-genetics system for APV. A minigenome system was used to optimize the expression of the nucleoprotein, phosphoprotein, M2 and large polymerase proteins when transfected into Vero cells under the control of the bacteriophage T7 promoter. Subsequently, cDNA was transcribed from the virion RNA to make a...
Paper Details
Title
Development of a reverse-genetics system for Avian pneumovirus demonstrates that the small hydrophobic (SH) and attachment (G) genes are not essential for virus viability
Published Date
Nov 1, 2004
Journal
Volume
85
Issue
11
Pages
3219 - 3227
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Notes
History