Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
Abstract
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice...
Paper Details
Title
Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
Published Date
Nov 19, 2013
Journal
Volume
24
Issue
1
Pages
125 - 131
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