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Jin-Soo Kim
Seoul National University
253Publications
52H-index
10.9kCitations
Publications 253
Newest
Published on Apr 1, 2019in Nature Biotechnology 35.72
Daesik Kim12
Estimated H-index: 12
(Seoul National University),
Da-eun Kim3
Estimated H-index: 3
(Seoul National University)
+ 2 AuthorsJin-Soo Kim52
Estimated H-index: 52
(Seoul National University)
Adenine base editors1 enable efficient targeted adenine-to-guanine single nucleotide conversions to induce or correct point mutations in human cells, animals, and plants1–4. Here we present a modified version of Digenome-seq, an in vitro method for identifying CRISPR (clustered regularly interspaced short palindromic repeats)-induced double-strand breaks using whole-genome sequencing5–8, to assess genome-wide target specificity of adenine base editors. To produce double-strand breaks at sites co...
8 Citations Source Cite
Published on Feb 15, 2019in The EMBO Journal 10.56
Viktorija Globyte2
Estimated H-index: 2
(Delft University of Technology),
Seung Hwan Lee3
Estimated H-index: 3
(Delft University of Technology)
+ 2 AuthorsChirlmin Joo3
Estimated H-index: 3
(Delft University of Technology)
Abstract The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) nuclease has been widely applied in genetic engineering. Despite its importance in genome editing, aspects of the precise molecular mechanism of Cas9 activity remain ambiguous. In particular, because of the lack of a method with high spatio‐temporal resolution, transient interactions between Cas9 and DNA could not be reliably investigated. It therefore remains controversial how Cas9 searches for protospacer adjacent motif (PAM) sequences. ...
2 Citations Source Cite
Published on Jan 1, 2019in Molecular Therapy 7.01
Dong Hyun Jo12
Estimated H-index: 12
(Seoul National University Hospital),
Taeyoung Koo14
Estimated H-index: 14
(Kyung Hee University)
+ 3 AuthorsJeong Hun Kim38
Estimated H-index: 38
(Seoul National University)
Genome editing with CRISPR systems provides an unprecedented opportunity to modulate cellular responses in pathological conditions by inactivating undruggable targets, such as transcription factors. Previously, we demonstrated that the smallest Cas9 ortholog characterized to date, from Campylobacter jejuni (CjCas9) targeted to Hif1a and delivered in an adeno-associated virus (AAV) vector, effectively suppressed pathological choroidal neovascularization in the mouse retina. Before implementation ...
1 Citations Source Cite
Published on Dec 12, 2018in Nano Letters 12.08
Hyomin Lee7
Estimated H-index: 7
(Jeju National University),
Jihye Choi2
Estimated H-index: 2
(Seoul National University)
+ 7 AuthorsSung Jae Kim20
Estimated H-index: 20
(Seoul National University)
The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapi...
2 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Jungjoon K. Lee2
Estimated H-index: 2
,
Euihwan Jeong5
Estimated H-index: 5
(Seoul National University)
+ 8 AuthorsSeokjoong Kim14
Estimated H-index: 14
The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human cells. Here, we develop a directed evolution approach in E. coli to obtain Sniper-Cas9, which shows high specificities without killing on-target activities in human cells. Unlike other engineered Cas...
18 Citations Source Cite
Published on Dec 1, 2018in Genome Research 10.10
Daesik Kim12
Estimated H-index: 12
(Seoul National University),
Jin-Soo Kim52
Estimated H-index: 52
(Seoul National University)
2 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Yongmoon Jeon6
Estimated H-index: 6
(Korea Institute of Science and Technology),
You Hee Choi1
Estimated H-index: 1
(Gwangju Institute of Science and Technology)
+ 10 AuthorsJin-Soo Kim52
Estimated H-index: 52
(Seoul National University)
Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to type II CRISPR–Cas9 for genome editing. Previous studies have revealed that Cas12a has unique features distinct from Cas9, but the detailed mechanisms of target searching and DNA cleavage by Cas12a are still unclear. Here, we directly observe this entire process by using single-molecule fluorescence assays to study Cas12a from Acidaminococcus sp. (AsCas12a). We det...
8 Citations Source Cite
Published on Dec 1, 2018in BMC Bioinformatics 2.21
Gue-Ho Hwang2
Estimated H-index: 2
(Hanyang University),
Jeongbin Park8
Estimated H-index: 8
(Charité)
+ 7 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
Background As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing tools will be very useful for gene correction because they can produce highly specific DNA substitutions without the introduction of any donor DNA, but dedicated web-based tools to facilitate...
3 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Taeyoung Koo14
Estimated H-index: 14
,
Sung Wook Park7
Estimated H-index: 7
(Seoul National University)
+ 6 AuthorsJin-Soo Kim52
Estimated H-index: 52
(Seoul National University)
LbCpf1, derived from Lachnospiraceae bacterium ND2006, is a CRISPR RNA-guided endonuclease and holds promise for therapeutic applications. Here we show that LbCpf1 can be used for therapeutic gene editing in a mouse model of age-related macular degeneration (AMD). The intravitreal delivery of LbCpf1, targeted to two angiogenesis-associated genes encoding vascular endothelial growth factor A (Vegfa) and hypoxia inducing factor 1a (Hif1a), using adeno-associated virus, led to efficient gene disrup...
4 Citations Source Cite
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