Jin-Soo Kim
Seoul National University
172Publications
51H-index
10.1kCitations
Publications 172
Newest
Published on Feb 15, 2019in The EMBO Journal 10.56
Viktorija Globyte2
Estimated H-index: 2
(Delft University of Technology),
Seung Hwan Lee2
Estimated H-index: 2
(Delft University of Technology)
+ 2 AuthorsChirlmin Joo3
Estimated H-index: 3
(Delft University of Technology)
Abstract The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) nuclease has been widely applied in genetic engineering. Despite its importance in genome editing, aspects of the precise molecular mechanism of Cas9 activity remain ambiguous. In particular, because of the lack of a method with high spatio‐temporal resolution, transient interactions between Cas9 and DNA could not be reliably investigated. It therefore remains controversial how Cas9 searches for protospacer adjacent motif (PAM) sequences. ...
2 Citations Source Cite
Published on Jan 1, 2019in Molecular Therapy 7.01
Dong Hyun Jo12
Estimated H-index: 12
(Seoul National University Hospital),
Taeyoung Koo14
Estimated H-index: 14
(Kyung Hee University)
+ 3 AuthorsJeong Hun Kim35
Estimated H-index: 35
(Seoul National University)
Genome editing with CRISPR systems provides an unprecedented opportunity to modulate cellular responses in pathological conditions by inactivating undruggable targets, such as transcription factors. Previously, we demonstrated that the smallest Cas9 ortholog characterized to date, from Campylobacter jejuni (CjCas9) targeted to Hif1a and delivered in an adeno-associated virus (AAV) vector, effectively suppressed pathological choroidal neovascularization in the mouse retina. Before implementation ...
1 Citations Source Cite
Published on Jan 1, 2018in International Journal of Ophthalmology 1.17
Jin-Soo Kim51
Estimated H-index: 51
,
Jung Yeol Choi1
Estimated H-index: 1
+ 2 AuthorsYoung Keun Han7
Estimated H-index: 7
Published on Dec 1, 2018in BMC Bioinformatics 2.21
Gue-Ho Hwang2
Estimated H-index: 2
(Hanyang University),
Jeongbin Park7
Estimated H-index: 7
(Charité)
+ 7 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
Background As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing tools will be very useful for gene correction because they can produce highly specific DNA substitutions without the introduction of any donor DNA, but dedicated web-based tools to facilitate...
3 Citations Source Cite
Published on Jul 30, 2018in Nature Biotechnology 35.72
Hyun Ji Lee2
Estimated H-index: 2
,
Jin-Soo Kim51
Estimated H-index: 51
Cas9 can induce extensive on-target damage, including large deletions, inversions, and insertions.
2 Citations Source Cite
Published on Oct 1, 2018in Nature Medicine 32.62
Huiyun Seo , Jin-Soo Kim51
Estimated H-index: 51
(Seoul National University)
Base editors function in mouse fetuses and in the livers of adult mice to treat a genetic disorder.
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Kyungjin Min2
Estimated H-index: 2
(UPRRP College of Natural Sciences),
Hyunjun Yoon1
Estimated H-index: 1
(UPRRP College of Natural Sciences)
+ 4 AuthorsJin-Soo Kim51
Estimated H-index: 51
(UPRRP College of Natural Sciences)
Abstract Clustered regularly interspaced short palindromic repeats (CRISPRs) from Prevotella and Francisella 1 (Cpf1) are RNA-guided endonucleases that produce cohesive double-stranded breaks in DNA by specifically recognizing thymidine-rich protospacer-adjacent motif (PAM) sequences. Cpf1 is emerging as a powerful genome-editing tool. Despite previous structural studies on various Cpf1 proteins, the apo-structure of Cpf1 remains unknown. In the present study, we determined the solution structur...
2 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Taeyoung Koo14
Estimated H-index: 14
(University of Science and Technology, Sana'a),
Sung Wook Park13
Estimated H-index: 13
(Seoul National University)
+ 6 AuthorsJin-Soo Kim51
Estimated H-index: 51
(University of Science and Technology, Sana'a)
LbCpf1, derived from Lachnospiraceae bacterium ND2006, is a CRISPR RNA-guided endonuclease and holds promise for therapeutic applications. Here we show that LbCpf1 can be used for therapeutic gene editing in a mouse model of age-related macular degeneration (AMD). The intravitreal delivery of LbCpf1, targeted to two angiogenesis-associated genes encoding vascular endothelial growth factor A (Vegfa) and hypoxia inducing factor 1a (Hif1a), using adeno-associated virus, led to efficient gene disrup...
4 Citations Source Cite
Published on Jul 16, 2018in PLOS Biology 9.16
Jin-Soo Kim51
Estimated H-index: 51
(Seoul National University),
Jacob E. Corn28
Estimated H-index: 28
(University of California, Berkeley)
Fast-moving, competitive fields often inadvertently duplicate research. In a research environment that values being first over being robust, this results in one manuscript “scooping” ongoing research from other groups. Opportunities to demonstrate the solidity of a result through coincidental reproduction are thus lost. Here, two group leaders, one the scooper and one the scoopee, discuss their experiences under PLOS Biology’s new “complementary research” policy. In this case, submission of the ...
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Published on Jul 1, 2018in Nature plants 11.47
Beum-Chang Kang3
Estimated H-index: 3
(University of Science and Technology, Sana'a),
Jae-Young Yun2
Estimated H-index: 2
+ 5 AuthorsJin-Soo Kim51
Estimated H-index: 51
(University of Science and Technology, Sana'a)
The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in...
10 Citations Source Cite
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