Jin-Soo Kim
Seoul National University
Publications 195
#1Dong Hyun Jo (Seoul National University Hospital)H-Index: 13
#2Dong Woo SongH-Index: 1
Last.Jungmin Lee (Handong Global University)H-Index: 4
view all 13 authors...
Leber congenital amaurosis (LCA), one of the leading causes of childhood-onset blindness, is caused by autosomal recessive mutations in several genes including RPE65. In this study, we performed CRISPR-Cas9–mediated therapeutic correction of a disease-associated nonsense mutation in Rpe65 in rd12 mice, a model of human LCA. Subretinal injection of adeno-associated virus carrying CRISPR-Cas9 and donor DNA resulted in >1% homology-directed repair and ~1.6% deletion of the pathogenic stop codon in ...
1 CitationsSource
#1Heon Seok KimH-Index: 6
#2You Kyeong Jeong (Hanyang University)H-Index: 1
Last.Sangsu Bae (Hanyang University)H-Index: 15
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Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5–7) and in a confined TC*N sequence context. Adenine base editor–induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-speci...
7 CitationsSource
#1Choongil Lee (SNU: Seoul National University)H-Index: 5
#2Dong Hyun Jo (Seoul National University Hospital)H-Index: 13
Last.Sangsu Bae (Hanyang University)H-Index: 15
view all 9 authors...
A nonsense mutation is a substitutive mutation in a DNA sequence that causes a premature termination during translation and produces stalled proteins, resulting in dysfunction of a gene. Although it usually induces severe genetic disorders, there are no definite methods for inducing read through of premature termination codons (PTCs). Here, we present a targeted tool for bypassing PTCs, named CRISPR-pass, that uses CRISPR-mediated adenine base editors. CRISPR-pass, which should be applicable to ...
2 CitationsSource
#1P. J. BonofigloH-Index: 2
#2Jay AndersonH-Index: 49
Last.Eli ParkeH-Index: 7
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CpG-DNA activates the host immune system to resist bacterial infections. In this study, we examined the protective effect of CpG-DNA in mice against Escherichia coli (E. coli) K1 infection. Administration of CpG-DNA increased the survival of mice after E. coli K1 infection, which reduces the numbers of bacteria in the organs. Pre-injection of mice with CpG-DNA before E. coli K1 infection increased the levels of the complement C3 but not C3a and C3b. The survival of the mice after E. coli K1 infe...
#1Marion Gruffaz (SC: University of Southern California)H-Index: 1
#2Hongfeng Yuan (SC: University of Southern California)H-Index: 6
Last.Shou-Jiang GaoH-Index: 41
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ABSTRACT The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, among which the cancer-vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) screening in a unique model of matched primary and...
#1Daesik Kim (SNU: Seoul National University)H-Index: 14
#2Da-eun Kim (SNU: Seoul National University)H-Index: 3
Last.Jin-Soo Kim (SNU: Seoul National University)H-Index: 53
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Adenine base editors1 enable efficient targeted adenine-to-guanine single nucleotide conversions to induce or correct point mutations in human cells, animals, and plants1–4. Here we present a modified version of Digenome-seq, an in vitro method for identifying CRISPR (clustered regularly interspaced short palindromic repeats)-induced double-strand breaks using whole-genome sequencing5–8, to assess genome-wide target specificity of adenine base editors. To produce double-strand breaks at sites co...
14 CitationsSource
#1Viktorija Globyte (TU Delft: Delft University of Technology)H-Index: 3
#2Seung Hwan Lee (TU Delft: Delft University of Technology)H-Index: 10
Last.Chirlmin Joo (TU Delft: Delft University of Technology)H-Index: 23
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9 CitationsSource
#1Dong Hyun Jo (Seoul National University Hospital)H-Index: 13
#2Taeyoung Koo (Kyung Hee University)H-Index: 15
Last.KimJi-Young (SNU: Seoul National University)H-Index: 141
view all 6 authors...
Genome editing with CRISPR systems provides an unprecedented opportunity to modulate cellular responses in pathological conditions by inactivating undruggable targets, such as transcription factors. Previously, we demonstrated that the smallest Cas9 ortholog characterized to date, from Campylobacter jejuni (CjCas9) targeted to Hif1a and delivered in an adeno-associated virus (AAV) vector, effectively suppressed pathological choroidal neovascularization in the mouse retina. Before implementation ...
3 CitationsSource