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Jin-Soo Kim
Seoul National University
253Publications
52H-index
10.9kCitations
Publications 253
Newest
#1Choongil Lee (SNU: Seoul National University)H-Index: 5
#2Dong Hyun Jo (Seoul National University Hospital)H-Index: 12
Last.Sangsu Bae (Hanyang University)H-Index: 13
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A nonsense mutation is a substitutive mutation in a DNA sequence that causes a premature termination during translation and produces stalled proteins, resulting in dysfunction of a gene. Although it usually induces severe genetic disorders, there are no definite methods for inducing read through of premature termination codons (PTCs). Here, we present a targeted tool for bypassing PTCs, named CRISPR-pass, that uses CRISPR-mediated adenine base editors. CRISPR-pass, which should be applicable to ...
CpG-DNA activates the host immune system to resist bacterial infections. In this study, we examined the protective effect of CpG-DNA in mice against Escherichia coli (E. coli) K1 infection. Administration of CpG-DNA increased the survival of mice after E. coli K1 infection, which reduces the numbers of bacteria in the organs. Pre-injection of mice with CpG-DNA before E. coli K1 infection increased the levels of the complement C3 but not C3a and C3b. The survival of the mice after E. coli K1 infe...
#1Marion GruffazH-Index: 1
#2Hongfeng YuanH-Index: 6
Last.Shou-Jiang GaoH-Index: 39
view all 9 authors...
ABSTRACT The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, among which the cancer-vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) screening in a unique model of matched primary and...
#1Daesik Kim (SNU: Seoul National University)H-Index: 12
#2Da-eun Kim (SNU: Seoul National University)H-Index: 3
Last.Jin-Soo Kim (SNU: Seoul National University)H-Index: 52
view all 5 authors...
Adenine base editors1 enable efficient targeted adenine-to-guanine single nucleotide conversions to induce or correct point mutations in human cells, animals, and plants1–4. Here we present a modified version of Digenome-seq, an in vitro method for identifying CRISPR (clustered regularly interspaced short palindromic repeats)-induced double-strand breaks using whole-genome sequencing5–8, to assess genome-wide target specificity of adenine base editors. To produce double-strand breaks at sites co...
#1Viktorija Globyte (TU Delft: Delft University of Technology)H-Index: 2
#2Seung Hwan Lee (TU Delft: Delft University of Technology)H-Index: 3
Last.Chirlmin Joo (TU Delft: Delft University of Technology)H-Index: 3
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Abstract The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) nuclease has been widely applied in genetic engineering. Despite its importance in genome editing, aspects of the precise molecular mechanism of Cas9 activity remain ambiguous. In particular, because of the lack of a method with high spatio‐temporal resolution, transient interactions between Cas9 and DNA could not be reliably investigated. It therefore remains controversial how Cas9 searches for protospacer adjacent motif (PAM) sequences. ...
#1Dong Hyun Jo (Seoul National University Hospital)H-Index: 12
#2Taeyoung Koo (Kyung Hee University)H-Index: 14
Last.Jeong Hun Kim (SNU: Seoul National University)H-Index: 32
view all 6 authors...
Genome editing with CRISPR systems provides an unprecedented opportunity to modulate cellular responses in pathological conditions by inactivating undruggable targets, such as transcription factors. Previously, we demonstrated that the smallest Cas9 ortholog characterized to date, from Campylobacter jejuni (CjCas9) targeted to Hif1a and delivered in an adeno-associated virus (AAV) vector, effectively suppressed pathological choroidal neovascularization in the mouse retina. Before implementation ...
#1Hyomin Lee (Jeju National University)H-Index: 7
#2Jihye Choi (SNU: Seoul National University)H-Index: 2
Last.Sung Jae Kim (SNU: Seoul National University)H-Index: 20
view all 10 authors...
The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapi...
#1Jungjoon K. LeeH-Index: 2
#2Euihwan Jeong (SNU: Seoul National University)H-Index: 5
Last.Seokjoong KimH-Index: 14
view all 11 authors...
The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human cells. Here, we develop a directed evolution approach in E. coli to obtain Sniper-Cas9, which shows high specificities without killing on-target activities in human cells. Unlike other engineered Cas...
#1Yongmoon Jeon (KIST: Korea Institute of Science and Technology)H-Index: 6
#2You Hee Choi (Gwangju Institute of Science and Technology)H-Index: 1
Last.Jin-Soo Kim (SNU: Seoul National University)H-Index: 52
view all 13 authors...
Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to type II CRISPR–Cas9 for genome editing. Previous studies have revealed that Cas12a has unique features distinct from Cas9, but the detailed mechanisms of target searching and DNA cleavage by Cas12a are still unclear. Here, we directly observe this entire process by using single-molecule fluorescence assays to study Cas12a from Acidaminococcus sp. (AsCas12a). We det...
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