Immunopurification and characterization of a rape (Brassica napus L.) seedling lipase

Volume: 12, Issue: 21, Pages: 3224 - 3234
Published: May 22, 2013
Abstract
Lipase or triacylglycerol acylhydrolase (E.C.3.1.1.3) was purified to homogeneity from rapeseed-germinated cotyledons ( Brassica napus null L.). The purification scheme involved homogenization, centrifugation, ultracentrifugation and affinity chromatography using polyclonal antibodies raised against porcine pancreatic lipase. The purified rapeseed lipase was homogenous and did not contain contaminating proteins detectable by SDS-PAGE and HPLC...
Paper Details
Title
Immunopurification and characterization of a rape (Brassica napus L.) seedling lipase
Published Date
May 22, 2013
Volume
12
Issue
21
Pages
3224 - 3234
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