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Hatem Belguith
Tunis University
16Publications
5H-index
75Citations
Publications 16
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Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are encoded by extremely complex gene families, and appear to fulfil many different biological functions. Although they are present in all types of organisms, available information on plant lipases is still very limited, as compared to their bacterial and animal counterparts. A full-length clone, BnLIP, encoding a putative lipase, has been isolated by PCR amplification of ...
#1Abdelmonaem Messaoudi (Tunis University)H-Index: 4
#2Hatem Belguith (Tunis University)H-Index: 5
Last.Jeannette Ben Hamida (Tunis University)H-Index: 10
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Background: blaVEB-1 is an integron-located extended-spectrum β-lactamase gene initially detected in Escherichia coli and Pseudomonas aeruginosa strains from southeast Asia. Several recent studies have reported that VEB-1-positive strains are highly resistant to ceftazidime, cefotaxime and aztreonam antibiotics. One strategy to overcome resistance involves administering antibiotics together with β-lactamase inhibitors during the treatment of infectious diseases. During this study, four VEB-1 β-l...
Lipases (triacylglycerol acyl hydrolass EC 3.1.1.3) are defined as enzymes that catalyse the hydrolysis of triacylglycerols, releasing long-chain fatty acids. Germinating oilseeds have been explored as a possible source of lipases for biotechnological processing of oils and fats. However, purification and sensitive assays to detect a true lipase activity in plant cellular homogenates and culture media are required. The main aim of this study was to design a convenient, specific, sensitive and co...
#1Hatem BelguithH-Index: 5
#2S. FattouchH-Index: 2
Last.Hamida J BenH-Index: 1
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Lipase or triacylglycerol acylhydrolase (E.C.3.1.1.3) was purified to homogeneity from rapeseed-germinated cotyledons ( Brassica napus L.). The purification scheme involved homogenization, centrifugation, ultracentrifugation and affinity chromatography using polyclonal antibodies raised against porcine pancreatic lipase. The purified rapeseed lipase was homogenous and did not contain contaminating proteins detectable by SDS-PAGE and HPLC analysis. The specific activity of the purified preparatio...
#1Abdelmonaem Messaoudi (Tunis University)H-Index: 4
#2Hatem Belguith (Tunis University)H-Index: 5
Last.Jeannette Ben Hamida (Tunis University)H-Index: 10
view all 4 authors...
Lipase enzymes play an important role in lipid metabolism and are produced by a variety of species. Compared with animal, bacterial and fungal, little is known about plant lipases. Although lipases belong to many different protein families, they have the same architecture, the ?/?-hydrolase fold and a conserved active site signature, the Gly-Xaa-Ser-Xaa-Gly motif. Several studies on enzymatic activity and interfacial activation phenomenon of lipases confirm the presence of consensus sequence and...
Triacylglycerol lipases have been thoroughly characterized in mammals and microorganisms. By contrast, very little is known about plant lipases. In this investigation, a homology model of Arabidopsis thaliana lipase (NP_179126) was constructed using a human gastric lipase (PDB ID: 1HLG), as a template for model building. This model was then assessed for stereochemical quality and side chain environment. Natural substrates: tributyrin, trioctanoin and triolen were docked into the model to investi...
There has been a consistent increase in the search for alternative and efficient compound for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Garlic offers a promising alternative for food safety and bioconservation. Filter sterilized, aqueous garlic extract was tested for ability to inhibit the growth of some isolated Salmonella serovars. The fresh aqueous garlic extract (A.G.E., 57.1% (w/v), containing 324 µg/ml allicin) inhibited the growth and ki...
#1Hatem BelguithH-Index: 5
#2F. KthiriH-Index: 3
Last.A. LadoulsiH-Index: 2
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There has been a consistent increase in the search for alternative and efficient compound for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Garlic offers a promising alternative for food safety and bioconservation. Filter sterilized, aqueous garlic extract was tested for ability to inhibit the growth of some isolated
#1Hatem BelguithH-Index: 5
#2F. KthiriH-Index: 3
Last.Ameni LandoulsiH-Index: 3
view all 6 authors...
El efecto sobre el crecimiento de Salmonella hadar fue investigado utilizando un medio liquido esteril fresco (Pronadisa, Hispanlab) conteniendo el extracto acuoso de ajo (EAA) en diferentes concentraciones (0, 11, 12 y 13 mg/ml). El extracto de ajo anadido con estas concentraciones tuvo un efecto bacteriostatico sobre Salmonella hadar. La prueba serovar revelo un patron de inhibicion caracterizado por: (i) una fase de inhibicion transitoria cuya duracion fue proporcional a la concentracion de E...
L.). The purification scheme involved homogenization, centrifugation, ultracentrifugation and affinity chromatography using polyclonal antibodies raised against porcine pancreatic lipase. The purified rapeseed lipase was homogenous and did not contain contaminating proteins detectable by SDS-PAGE and HPLC analysis. The specific activity of the purified preparation was increased about 1950 times, with an overall yield of 35%. The rapeseed lipase was found to be a cytosoluble, glycosylated and hea...
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