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Determination of polyamine metabolome in plasma and urine by ultrahigh performance liquid chromatography–tandem mass spectrometry method: Application to identify potential markers for human hepatic cancer

Published on Aug 1, 2013in Analytica Chimica Acta5.26
· DOI :10.1016/j.aca.2013.06.044
Ran Liu10
Estimated H-index: 10
(SPU: Shenyang Pharmaceutical University),
Qing Li18
Estimated H-index: 18
(SPU: Shenyang Pharmaceutical University)
+ 3 AuthorsKaishun Bi28
Estimated H-index: 28
(SPU: Shenyang Pharmaceutical University)
Abstract
Abstract To evaluate the potential relationship between cancer and polyamine metabolome, a UHPLC–MS/MS method has been developed and validated for simultaneous determination of polyamine precursors, polyamines, polyamine catabolite in human plasma and urine. Polyamine precursors including l -ornithine, lysine, l -arginine and S-adenosyl- l -methionine; polyamines including 1,3-diaminopropane, putrescine, cadaverine, spermidine, spermine, agmatine, N-acetylputrescine, N-acetylspermine and N-acetylspermidine; polyamine catabolite including γ-aminobutyric acid had been determined. The analytes were extracted from plasma and urine samples by protein precipitation procedure, and then separated on a Shim-pack XR-ODS column with 0.05% heptafluorobutyric acid (HFBA) in methanol and 0.05% HFBA in water. The detection was performed on UHPLC–MS/MS system with turbo ion spray source in the positive ion and multiple reaction-monitoring mode. The limits of quantitation for all analytes were within 0.125–31.25 ng mL −1 in plasma and urine. The absolute recoveries of analytes from plasma and urine were all more than 50%. By means of the method developed, the plasma and urine samples from hepatic cancer patients and healthy age-matched volunteers had been successfully determined. Results showed that putrescine and spermidine in hepatic cancerous plasma were significant higher than those in healthy ones, while spermidine, spermine and N-acetylspermidine in hepatic cancerous urine were significant higher than those in healthy ones. The methods demonstrated the changes of polyamine metabolome occurring in plasma and urine from human subjects with hepatic cancer. It could be a powerful manner to indicate and treat hepatic cancer in its earliest indicative stages.
  • References (25)
  • Citations (43)
References25
Newest
#1Qian Wang (SPU: Shenyang Pharmaceutical University)H-Index: 3
#2Yixiang Wang (SPU: Shenyang Pharmaceutical University)H-Index: 4
Last.Qing Li (SPU: Shenyang Pharmaceutical University)H-Index: 18
view all 8 authors...
#1Ran Liu (SPU: Shenyang Pharmaceutical University)H-Index: 10
#2Kaishun Bi (SPU: Shenyang Pharmaceutical University)H-Index: 28
Last.Qing Li (SPU: Shenyang Pharmaceutical University)H-Index: 18
view all 6 authors...
#1Ran Liu (SPU: Shenyang Pharmaceutical University)H-Index: 10
#2Ying Jia (SPU: Shenyang Pharmaceutical University)H-Index: 7
Last.Qing Li (SPU: Shenyang Pharmaceutical University)H-Index: 18
view all 7 authors...
#1Xin-Qing PengH-Index: 1
#2Fei Wang (Tianjin Medical University)H-Index: 3
Last.Wei-Ming Zhang (Tianjin Medical University)H-Index: 1
view all 4 authors...
#1Ling Li (PRC: China Medical University (PRC))H-Index: 2
#2Kenji Hara (Fukuoka University)H-Index: 16
Last.Yanfang Wang (PRC: China Medical University (PRC))H-Index: 2
view all 7 authors...
Cited By43
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#1Hyung-Min Kim (SNU: Seoul National University)H-Index: 28
#2Nguyen Phuoc Long (SNU: Seoul National University)H-Index: 6
Last.Sung Won Kwon (SNU: Seoul National University)H-Index: 25
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#1Tünde Kovács (University of Debrecen)H-Index: 8
#2Edit Mikó (University of Debrecen)H-Index: 8
Last.Patrik Kovács (University of Debrecen)H-Index: 2
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#1Erik Hanff (MHH: Hannover Medical School)H-Index: 9
#2Alexander Bollenbach (MHH: Hannover Medical School)H-Index: 3
Last.Dimitrios Tsikas (MHH: Hannover Medical School)H-Index: 50
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#1Sarah S. Dinges (Charité)
#2Annika Hohm (Harvard University)H-Index: 2
Last.Leo K. Cheng (Harvard University)H-Index: 36
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#1Jing Song (PRC: China Medical University (PRC))
#2Zhongyan Shan (PRC: China Medical University (PRC))H-Index: 33
Last.Weiping Teng (PRC: China Medical University (PRC))H-Index: 27
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