Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system
Abstract
Technologies allowing for specific regulation of endogenous genes are valuable for the study of gene functions and have great potential in therapeutics. We created the CRISPR-on system, a two-component transcriptional activator consisting of a nuclease-dead Cas9 (dCas9) protein fused with a transcriptional activation domain and single guide RNAs (sgRNAs) with complementary sequence to gene promoters. We demonstrate that CRISPR-on can efficiently...
Paper Details
Title
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system
Published Date
Aug 27, 2013
Journal
Volume
23
Issue
10
Pages
1163 - 1171
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