A genetically engineered prime-boost vaccination strategy for oculonasal delivery with poly(d,l-lactic-co-glycolic acid) microparticles against infection of turkeys with avian Metapneumovirus

In this study we demonstrated the use of an oculonasally delivered poly(D,L-lactic-co-glycolic acid) microparticle (PLGA-MP)-based and genetically engineered vaccination strategy in the avian system. An avian Metapneumovirus (aMPV) fusion (F) protein-encoding plasmid vaccine and the corresponding recombinant protein vaccine were produced and bound to or encapsulated by PLGA-MP, respectively. The PLGA-MP as the controlled release system was shown...