Match!

Antifungal evaluation of Ocimum sanctum essential oil against fungal deterioration of raw materials of Rauvolfia serpentina during storage

Published on Feb 1, 2013in Industrial Crops and Products4.19
· DOI :10.1016/j.indcrop.2012.12.006
Ashok Kumar37
Estimated H-index: 37
(BHU: Banaras Hindu University),
Nawal Kishore Dubey30
Estimated H-index: 30
(BHU: Banaras Hindu University),
Sharad Srivastava11
Estimated H-index: 11
(NBRI: National Botanical Research Institute)
Cite
Abstract
Abstract Raw materials (roots) of Rauvolfia serpentina were found associated with various storage moulds. During mycological screening, total 887 fungal isolates were recovered and among them Aspergillus flavus exhibited the highest relative density (35.74%). The essential oil (EO) of Ocimum sanctum was isolated through Clevenger's hydro-distillation apparatus and investigated against A. flavus LHPRS7, isolated from raw materials. The GC–MS analysis of EO showed 41 considerable peaks. Eugenol (61.30%) was found as major component followed by β-Caryophyllene (11.89%) and Germacrene D (9.14%). The minimum inhibitory concentration (MIC) of O. Sanctum against A. flavus was found at 0.3 μl ml −1 while aflatoxin B 1 production was completely checked at 0.2 μl ml −1 . The O. sanctum EO exhibited broad fungitoxic spectrum and also found efficacious in reducing fungal incidence during in vivo study. The EO reduces the number of A. flavus isolates up to 62.94, 67.87 and 74.01% fumigated at concentration 0.3, 0.5 and 1.0 μl ml −1 respectively. The HPTLC (high performance thin layer chromatography) analysis of raw materials in in vivo study reveals that alkaloid ajmaline was firmly degraded by fungal contamination while in the samples fumigated with O. sanctum EO, ajmaline was protected from fungal degradation due to antifungal efficacy of EO. The prospects of exploitation of O. sanctum EO as acceptable plant based additive in qualitative as well as quantitative control of biodeterioration of herbal raw materials have been discussed.
  • References (43)
  • Citations (9)
Cite
References43
Newest
Published on Apr 9, 2012in Journal of Agricultural Science and Technology0.83
Vivek K. Bajpai28
Estimated H-index: 28
(Yeungnam University),
Sun Chul Kang33
Estimated H-index: 33
(Daegu University)
This study was carried out to evaluate the in vitro and in vivo antifungal efficacy of essential oil and extracts derived from the flower and leaves of Magnolia liliflora , respectively, against plant pathogenic fungi. The oil (750 µg disc -1 ) and various leaf extracts such as hexane, chloroform, ethyl acetate and methanol (1,500 µg disc -1 ) revealed promising antifungal effects against Botrytis cinerea KACC 40573, Colletotrichum capsici KACC 40978 , Fusarium oxysporum KACC 41083 , Fusarium so...
Published on Mar 1, 2012in Industrial Crops and Products4.19
Prashant Kumar Mishra14
Estimated H-index: 14
(BHU: Banaras Hindu University),
Ravindra Shukla18
Estimated H-index: 18
(BHU: Banaras Hindu University)
+ 2 AuthorsNawal Kishore Dubey30
Estimated H-index: 30
(BHU: Banaras Hindu University)
Abstract The study deals with evaluation of antifungal and antiaflatoxigenic Caesulia axillaris Roxb. essential oil (EO) against herbal raw materials deteriorating fungi and its free radical scavenging activity. During mycoflora analysis these herbal raw materials were found to be severely contaminated by different fungi and aflatoxins. A total of nine different fungal species were isolated from three herbal raw materials. Aspergillus flavus LHPtc was recorded as the highest aflatoxin B1 produci...
Published on Dec 1, 2010in Research in Microbiology2.65
Amber Khan13
Estimated H-index: 13
(Jamia Millia Islamia),
Aijaz Ahmad19
Estimated H-index: 19
(Jamia Millia Islamia)
+ 4 AuthorsNikhat Manzoor21
Estimated H-index: 21
(Jamia Millia Islamia)
Abstract The increasing incidence of drug-resistant pathogens and host toxicity of existing antifungals attracts attention toward the efficacy of natural products as antifungals in mucocutaneous infections and combinational therapies. The composition and antifungal activity of the essential oil obtained from Ocimum sanctum (OSEO) was studied. On GC–MS analysis, OSEO showed a high content of methyl chavicol (44.63%) and linalool (21.84%). Antifungal activity of OSEO and its two main constituents ...
Published on Mar 1, 2010in Food Chemistry5.40
D. Runyoro1
Estimated H-index: 1
(MUHAS: Muhimbili University of Health and Allied Sciences),
Olipa Ngassapa13
Estimated H-index: 13
(MUHAS: Muhimbili University of Health and Allied Sciences)
+ 3 AuthorsIoanna Chinou35
Estimated H-index: 35
(UoA: National and Kapodistrian University of Athens)
Abstract As part of ongoing research on Tanzanian plants used as edibles or spices, six samples of essential oils from four Ocimum species ( O. basilicum , O. kilimandscharicum , O. lamiifolium , O. suave ) were analyzed by GC and GC–MS. Eighty-one compounds, corresponding to 81.1–98.2% of the chemical components of the oils, were identified. Major compounds were either phenyl propane derivatives or terpenoids, including methyl eugenol, 1,8-cineole, camphor, bornyl acetate, germacrene-D, E-myrox...
Published on Feb 1, 2010in Food and Chemical Toxicology3.77
Ashok Kumar37
Estimated H-index: 37
(BHU: Banaras Hindu University),
Ravindra Shukla18
Estimated H-index: 18
(BHU: Banaras Hindu University)
+ 1 AuthorsNawal Kishore Dubey30
Estimated H-index: 30
(BHU: Banaras Hindu University)
Abstract The study deals with the efficacy of Ocimum sanctum essential oil (EO) and its major component, eugenol against the fungi causing biodeterioration of food stuffs during storage. O. sanctum EO and eugenol were found efficacious in checking growth of Aspergillus flavus NKDHV8; and, their minimum inhibitory concentrations (MICs) were recorded as 0.3 and 0.2 μl ml −1 , respectively. The O . sanctum EO and eugenol also inhibited the aflatoxin B 1 production completely at 0.2 and 0.1 μl ml −1...
Published on Oct 1, 2009in Journal of Scientific & Industrial Research0.73
Sharad Srivastava11
Estimated H-index: 11
,
Manjoosha Srivastava6
Estimated H-index: 6
+ 1 AuthorsA. K. S. Rawat7
Estimated H-index: 7
A simple, rapid, and selective HPTLC method has been established for separation and determination of curcumin, extracted from rhizomes of different Curcuma species, which are sold in herbal drug markets of India by the name of Amraharidra. Chromatography was performed on silica gel 60F 254 plates with chloroform: ethanol: acetic acid, 95: 4: 5 (v/v), as mobile phase. Detection and quantification were performed densitometrically at λ max = 260 nm with curcumin as external standard. This method wa...
Published on Sep 1, 2009in Industrial Crops and Products4.19
Martin Zabka5
Estimated H-index: 5
,
Roman Pavela30
Estimated H-index: 30
,
Ludmila Slezakova1
Estimated H-index: 1
Abstract Essential oils from 25 species of medicinal plants were tested as mycelial growth inhibitors against six important pathogenic and toxinogenic fungal species. An agar dilution method was used for determination of the inhibitory effect namely on Fusarium oxysporum , Fusarium verticillioides , Penicillium expansum , Penicillium brevicompactum , Aspergillus flavus and Aspergillus fumigatus . All essential oils used in our experiment evidently affected growth of these fungi. Minimum inhibito...
Ashok Kumar37
Estimated H-index: 37
(BHU: Banaras Hindu University),
Ravindra Shukla18
Estimated H-index: 18
(BHU: Banaras Hindu University)
+ 2 AuthorsNawal Kishore Dubey30
Estimated H-index: 30
(BHU: Banaras Hindu University)
Abstract A total of 14 odoriferous angiospermic essential oils were tested against the toxigenic strain of Aspergillus flavus . The essential oil of Thymus vulgaris L. showed highest antifungal efficacy. The thyme oil absolutely inhibited the mycelial growth of A. flavus at 0.7 μl ml − 1 and exhibited a broad fungitoxic spectrum against eight different food contaminating fungi viz. Fusarium oxysporum , Cladosporium herbarum , Curvularia lunata , Aspergillus terreus , Aspergillus niger , Aspergil...
Published on Oct 1, 2008in Microbial Ecology3.61
Pramod Kumar Singh22
Estimated H-index: 22
(BHU: Banaras Hindu University),
Bhawana Srivastava7
Estimated H-index: 7
(BHU: Banaras Hindu University)
+ 1 AuthorsN. K. Dubey9
Estimated H-index: 9
(BHU: Banaras Hindu University)
The paper explores fungal infection and aflatoxin B1 contamination of six medicinal plant samples viz. Adhatoda vasica Nees, Asparagus racemosus Linn., Evolvulus alsinoides Linn., Glycyrrhiza glabra Linn., Plumbago zeylanica Linn. and Terminalia chebula Retz. A total of 858 fungal isolates were detected from the raw materials. Maximum number of fungal isolates was detected from A. racemosus (228). The genus Aspergillus was found to be the most dominant genus causing infection to most of the raw ...
Published on Sep 1, 2008in Journal of Ethnopharmacology3.41
Mohaddese Mahboubi8
Estimated H-index: 8
,
Ghasem Haghi6
Estimated H-index: 6
Abstract Ethnopharmacological relevance The flowering aerial parts of Mentha pulegium L. (Labiatae) has been traditionally used for its antiseptic properties for treatment of infectious diseases. Aim of the study In order to validate its antiseptic properties with respect to traditional uses, we have screened the antimicrobial activity of flowering aerial parts of Mentha pulegium L. essential oil against different microorganisms. Materials and methods This oil was obtained using hydrodistillatio...
Cited By9
Newest
Published on Jul 1, 2019in Postharvest Biology and Technology3.93
V. Singh35
Estimated H-index: 35
(BHU: Banaras Hindu University),
Somenath Das2
Estimated H-index: 2
(BHU: Banaras Hindu University)
+ 2 AuthorsNawal Kishore Dubey30
Estimated H-index: 30
(BHU: Banaras Hindu University)
Abstract The study reports industrial significance of chemically characterized nanoencapuslated Ocimum sanctum essential oil (OSEO) against fungi contaminating herbal raw materials and aflatoxin B 1 secretion. The chitosan encapsulated OSEO exhibited enhanced antifungal and antiaflatoxigenic potential as compared to unencapsulated OSEO. The nanoencapsulated OSEO reduced the ergosterol content and enhanced the leakage of vital cellular ions along with the loss of 260 and 280 nm absorbing material...
Published on Jun 17, 2019in bioRxiv
Manu Shree (IITs: Indian Institutes of Technology), Ranjan Kumar Nanda5
Estimated H-index: 5
(International Centre for Genetic Engineering and Biotechnology),
Shyam K. Masakapalli8
Estimated H-index: 8
(IITs: Indian Institutes of Technology)
Tulsi (Ocimum species), the queen of herbs is a common ingredient in beverages with perceived health benefits. Recently published Ocimum genome highlighted the presence of several genes that contributes to important phytochemicals but a comprehensive metabolite profiling to study the water soluble metabolites of Ocimum is lacking. In this study, untargeted metabolic profiling of hot water extract of fresh and air dried leaves of O. basilicum, O. sanctum and O. kilimandscharicum species employing...
Published on Jul 3, 2017in International Journal of Food Properties1.40
Abdullah Ijaz Hussain18
Estimated H-index: 18
(GCUF: Government College University, Faisalabad),
Shahzad Ali Shahid Chatha12
Estimated H-index: 12
(GCUF: Government College University, Faisalabad)
+ 3 AuthorsMuhammad Ibrahim Lazhari1
Estimated H-index: 1
(Universiti Sains Malaysia)
ABSTRACTThe objective of the present research work was to evaluate the antioxidant, antimicrobial and antimalarial activities of essential oil and various extracts from O. sanctum. Gas chromatography-mass spectrometry analysis identified the following major compounds with their quantification as: eugenol (22.0%), β-elemene (19.2%), β-caryophyllene (19.1%), and Germacrene D (5.03%). HPLC analysis of O. sanctum extracts revealed that gallic acid, chlorogenic acid, p-hydroxy benzoic acid, caffeic a...
Published on Jun 16, 2017in International Journal of Molecular Sciences4.18
Qing Liu3
Estimated H-index: 3
,
Xiao Meng7
Estimated H-index: 7
+ 3 AuthorsHua-Bin Li42
Estimated H-index: 42
Infectious diseases caused by pathogens and food poisoning caused by spoilage microorganisms are threatening human health all over the world. The efficacies of some antimicrobial agents, which are currently used to extend shelf-life and increase the safety of food products in food industry and to inhibit disease-causing microorganisms in medicine, have been weakened by microbial resistance. Therefore, new antimicrobial agents that could overcome this resistance need to be discovered. Many spices...
Published on Sep 3, 2015in Frontiers in Microbiology4.26
Naveen Kumar Kalagatur6
Estimated H-index: 6
(Defence Food Research Laboratory),
Venkataramana Mudili8
Estimated H-index: 8
(Centre for Life)
+ 5 AuthorsVenkata L. Rao Putcha2
Estimated H-index: 2
(Centre for Life)
The present study was aimed to establish the antagonistic effects of Ocimum sanctum L. essential oil (OSEO) on growth and zearalenone (ZEA) production of Fusarium graminearum. GC-MS chemical profiling of OSEO revealed the existence of 43 compounds and the major compound was found to be eugenol (34.7%). DPPH free radical scavenging activity (IC50) of OSEO was determined to be 8.5µg/mL. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of OSEO on F. graminearum were...
Published on Sep 1, 2014in Industrial Crops and Products4.19
Jun Tian13
Estimated H-index: 13
(WHU: Wuhan University),
Xiaobin Zeng9
Estimated H-index: 9
(Guangdong Medical College)
+ 3 AuthorsYouwei Wang19
Estimated H-index: 19
(WHU: Wuhan University)
Abstract Essential oils are attracting attention owing to their potential as natural antimicrobial agents, and therefore, they find many applications in the food and pharmaceutical industries. This investigation aimed to evaluate the antifungal activity of Zanthoxylum molle essential oil (ZMEO) against Aspergillus flavus , a food contaminant. Gas chromatography–mass spectrometry detected 51 compounds representing 95.72% of the ZMEO. 2-Undecanone (26.81%), limonene (20.12%), and terpinen-4-ol (13...
Published on Mar 1, 2014in Phytomedicine4.18
Amber Khan13
Estimated H-index: 13
(Jamia Millia Islamia),
Aijaz Ahmad19
Estimated H-index: 19
(TUT: Tshwane University of Technology)
+ 2 AuthorsNikhat Manzoor21
Estimated H-index: 21
(Jamia Millia Islamia)
Abstract Candida albicans is an opportunistic human fungal pathogen which causes disease mainly in immunocompromised patients. Activity of hydrolytic enzymes is essential for virulence of C. albicans and so is the capacity of these cells to undergo transition from yeast to mycelial form of growth. Ocimum sanctum is cultivated worldwide for its essential oil which exhibits medicinal properties. This work evaluates the anti-virulence activity of O. sanctum essential oil (OSEO) on 22 strains of C. ...
Published on Jan 1, 2014in The Scientific World Journal
Thamilvaani Manaharan7
Estimated H-index: 7
,
Ramaraj Thirugnanasampandan5
Estimated H-index: 5
+ 3 AuthorsM.S. Kanthimathi4
Estimated H-index: 4
Antimetastatic and anti-inflammatory activities of Ocimum sanctum essential oil (OSEO) have been assessed in this study. OSEO at the concentration of 250 μg/mL and above showed a significant () decrease in the number of migrated cancer cells. In addition, OSEO at concentration of 250 μg/mL and above suppressed MMP-9 activity in lipopolysaccharide (LPS) induced inflammatory cells. A dose-dependent downregulation of MMP-9 expression was observed with the treatment of OSEO compared to the control. ...
Marc Cohen20
Estimated H-index: 20
(RMIT: RMIT University)
The predominant cause of global morbidity and mortality is lifestyle-related chronic diseases, many of which can be addressed through Ayurveda with its focus on healthy lifestyle practices and regular consumption of adaptogenic herbs. Of all the herbs used within Ayurveda, tulsi ( Ocimum sanctum Linn) is preeminent, and scientific research is now confirming its beneficial effects. There is mounting evidence that tulsi can address physical, chemical, metabolic and psychological stress through a u...
View next paperChemical composition of Ocimum basilicum L. essential oil and its efficacy as a preservative against fungal and aflatoxin contamination of dry fruits