Reusable Impedimetric Aptasensor

A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)63-/4-, using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the...