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Josep Lluis Acero Sánchez
Analytical chemistryAptamerChemistryCyclic voltammetryMonolayer
8Publications
6H-index
733Citations
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Publications 8
Newest
#1Ayman Ali SaeedH-Index: 4
Last. M. N. AbbasH-Index: 16
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Abstract Two different DNA (ERBB2c and CD24c) modified gold nanoparticles and graphene oxide loaded on glassy carbon electrodes were prepared for early detection of breast cancer markers by electrochemical detection of HER2. Comparative study of ERBB2c and CD24c for the detection was carried out. A “sandwich-type” detection strategy was employed in this electrochemical DNA biosensor and its response was measured by amperometric detection. The electrochemical signal enhancement achieved via gold ...
24 CitationsSource
#1Louis AdriaenssensH-Index: 14
Last. Pablo BallesterH-Index: 46
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Using a commercial instrument that integrates microfluidics with surface plasmon resonance (SPR) detection, we thermodynamically and kinetically characterise the interactions of water-soluble calix[4]pyrroles with surface-immobilized guests derived from pyridine N-oxide. This technique provides detailed information in real time and shows that, while thermodynamically similar, the binding process occurring with the surface-immobilised guests is kinetically very different to that in solution. Two ...
3 CitationsSource
An enzyme-linked oligonucleotide assay (ELONA) for quantification of mRNA expression of five genes involved in breast cancer, extracted from isolated rare tumour cells and amplified by multiplex ligation-dependent probe amplification (MLPA) is presented. In MLPA, a multiplex oligonucleotide ligation assay is combined with a PCR reaction in which all ligation products are amplified by use of a single primer pair. Biotinylated probes complementary to each of the target sequences were immobilised o...
5 CitationsSource
#1Olivier Y.F. HenryH-Index: 16
#2Alex FragosoH-Index: 26
Last. Ciara K. O'SullivanH-Index: 37
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We present the rapid prototyping of electrochemical sensor arrays integrated to microfluidicstowards the fabrication of integrated microsystems prototypes for point-of-carediagnostics. Rapid protot ...
35 CitationsSource
#2Eva BaldrichH-Index: 17
Last. Ciara K. O'SullivanH-Index: 37
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An electrochemical molecular beacon aptasensor for fast and sensitive assay of thrombin is developed and characterized. A bifunctional derivative of the thrombin-binding aptamer, 15-base long with a redox-active ferrocene moiety and a pendant hexanethiol linker group at the termini of the aptamer strand was immobilized on Au electrode surface. The electrochemical behavior of the aptasensor was studied using cyclic voltammetry (CV), chronoamperometry (CA), chronopotentiometry (CP), square-wave vo...
33 CitationsSource
Last. Ioanis KatakisH-Index: 24
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Self-assembled monolayers (SAM) were obtained on gold electrodes using thioctic esters of benzo[1,3]dioxinol.These SAMs present a group that can be electroactivated selectively and was used for the directed, reagentless, covalent patterning of proteins.The advantage of this moiety is that it allows electroactivation at low potentials increasing selectivity and reliability.In this study, the efficiency of this patterning system is examined.Cyclic voltammetry (CV) was used to confirm the electroac...
7 CitationsSource
A bifunctional derivative of the thrombin-binding aptamer with a redox-active Fc moiety and a thiol group at the termini of the aptamer strand was synthesized. The ferrocene-labeled aptamer thiol was self-assembled through S−Au bonding on a polycrystalline gold electrode surface and the surface was blocked with 2-mercaptoethanol to form a mixed monolayer. By use of a fluorescent molecular beacon, the effect of counterions on quadruplex formation was established. The aptamer-modified electrode wa...
416 CitationsSource
A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)63-/4-, using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer−thrombin complex, resulting in an increase of the int...
210 CitationsSource
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