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Cytotoxic potential of the preparations from Solanum trilobatum and the effect of sobatum on tumour reduction in mice.

Published on Dec 1, 1996in Cancer Letters6.51
· DOI :10.1016/S0304-3835(96)04463-1
P. Mohanan28
Estimated H-index: 28
,
K.S. Devi6
Estimated H-index: 6
(UoK: University of Kerala)
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Abstract
Abstract Plant Solanum trilobatum was washed, powdered and used for extraction. The lyophilized aqueous extracted portion was tested for in vitro cytotoxicity by tissue culture technique using L929 and Vero cells. Petroleum ether, chloroform, ethyl acetate and ethanol were used for extraction and the extracted portions were subjected to in vitro tissue culture studies. It was shown that petroleum ether extract induced remarkable cytotoxicity, when compared to all other extracts with an LD 50 of 7.0 μg in L929 and 5.8 μg in Vero cells. Further fractionated portions of petroleum ether extract (by adsorption chromatography) underwent tissue culture assay, and results suggest that petroleum ether/ethyl acetate (75:25) extractable portion is the most active fraction, named as sobatum, which induced an LD 50 of 7.0 μg in L929 and 7.5 μg in Vero cells. Sobatum significantly inhibit the peritoneal tumours induced by Dalton's lymphoma ascites (DLA) and Ehrlich ascites (EA) tumour cell. The effect was more prominent when sobatum was administered orally as evidenced from the increased percentage of life span. Sobatum, the partially purified portion of Solanum trilobatum , was again fractionated by column chromatography and all the residues were concentrated and crystallized from methanol, giving only one pure crystalline compound, that was identified as Betasitosterol by comparing with authentic sample.
  • References (13)
  • Citations (35)
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References13
Newest
P. Mohanan28
Estimated H-index: 28
,
K. Rathinam7
Estimated H-index: 7
,
K.S. Devi6
Estimated H-index: 6
(UoK: University of Kerala)
Abstract The plant Solanum trilobatum is mainly used for asthma, chronic febrile affections and difficult parturition. The active principle (Sobatum) obtained from the petroleum ether extract of the plant was proved as an anticancer agent by in vitro and in vivo experiments. Here, an effort was made to evaluate the induction of micronucleus by the Sobatum in the bone marrow of swiss mice. The micronucleus assay was conducted after 24 and 72 h of second administration of the Sobatum. The first se...
Published on Jun 1, 1994in Cancer Letters6.51
K.T. Mary1
Estimated H-index: 1
,
Kuttan Girija1
Estimated H-index: 1
,
Kuttan Ramadasan1
Estimated H-index: 1
Abstract Extracts from plants of Loranthaceae family collected from 15 host trees were screened for their cytocidal activity to tumour cells. Biologically active extracts prepared from Helicanthis elasticus grown on Mangifera indica and Citrus maxima (host trees) were partially purified by the gel filtration method. The purified material was found to be cytotoxic in vitro and reduced solid and ascites tumours in mice when given either simultaneously or prophylactically. The partially purified ma...
Published on Jan 1, 1993in Oncology Research4.63
E Marshall12
Estimated H-index: 12
,
Karen M. Holdaway11
Estimated H-index: 11
+ 3 AuthorsBruce C. Baguley59
Estimated H-index: 59
Published on May 1, 1990in Cancer Letters6.51
M.C. Unnikrishnan1
Estimated H-index: 1
,
R. Kuttan1
Estimated H-index: 1
Abstract Tumour reducing activity of extracts of eight commonly used spices in India were studied in mice transplanted intraperitoneally with Ehrlich ascites tumour. Oral administration of extracts of black pepper, asafoetida, pippali and garlic could increase the percentage of life span in these mice by 64.7%, 52.9%, 47% and 41.1%, respectively. However intraperitoneal administration of spice extracts did not produce any significant reduction in tumour growth except for sesame (38.8%). Garlic e...
Published on Apr 1, 1990in Journal of Ethnopharmacology3.41
Girija Kuttan34
Estimated H-index: 34
,
Dm Vasudevan24
Estimated H-index: 24
,
Ramadasan Kuttan40
Estimated H-index: 40
Abstract The effect of Iscador, a commercial preparation made from Viscum album was studied on several cell lines using in vitro tissue culture as well as tumor-bearing animals. Iscador was found to be cytotoxic to animal tumor cells such as Dalton's lymphoma ascites cells (DLA cells) and Ehrlich ascites cells in vitro and inhibited the growth of lung fibroblasts (LB cells), Chinese hamster ovary cells (CHO cells) and human nasopharyngeal carcinoma cells (KB cells) at very low concentrations. Mo...
Published on Nov 1, 1985in Cancer Letters6.51
Ramadasan Kuttan40
Estimated H-index: 40
,
P. Bhanumathy1
Estimated H-index: 1
+ 1 AuthorsM.C. George1
Estimated H-index: 1
Abstract Anticancer activity of the rhizomes of turmeric was evaluated in vitro using tissue culture methods and in vivo in mice using Dalton's lymphoma cells grown as ascites form. Turmeric extract inhibited the cell growth in Chinese Hamster Ovary (CHO) cells at a concentration of 0.4 mg/ml and was cytotoxic to lymphocytes and Dalton's lymphoma cells at the same concentration. Cytotoxic effect was found within 30 min at room temperature (30°C). The active constituent was found to be ‘curcumin’...
Published on Mar 1, 1971
Jonathan L. Hartwell1
Estimated H-index: 1
Published on May 1, 1983in Journal of Cancer Research and Clinical Oncology3.33
Martin R. Berger37
Estimated H-index: 37
(DKFZ: German Cancer Research Center),
D. Schmähl30
Estimated H-index: 30
(DKFZ: German Cancer Research Center)
The effect of Iscador was tested in a total of four tumor models involving some 214 rats and 93 mice. Although several doses of the agent were used, no significant antitumor effect was observed.
Frank M. Schabel34
Estimated H-index: 34
(Southern Research Institute),
Daniel P. Griswold22
Estimated H-index: 22
(Southern Research Institute)
+ 2 AuthorsLloyd Hh6
Estimated H-index: 6
(Southern Research Institute)
Cited By35
Newest
Published on Mar 12, 2019in Natural Products and Bioprospecting
Joseph Sakah Kaunda1
Estimated H-index: 1
(CAS: Chinese Academy of Sciences),
Ying-Jun Zhang25
Estimated H-index: 25
(CAS: Chinese Academy of Sciences)
Over the past 30 years, the genus Solanum has received considerable attention in chemical and biological studies. Solanum is the largest genus in the family Solanaceae, comprising of about 2000 species distributed in the subtropical and tropical regions of Africa, Australia, and parts of Asia, e.g., China, India and Japan. Many of them are economically significant species. Previous phytochemical investigations on Solanum species led to the identification of steroidal saponins, steroidal alkaloid...
Published on Dec 1, 2018in Scientific Reports4.01
Adil Lateef , Sudheesh K. Prabhudas2
Estimated H-index: 2
,
Purushothaman Natarajan3
Estimated H-index: 3
Solanum trilobatum L. is an important medicinal plant in traditional Indian system of medicine belonging to Solanaceae family. However, non-availability of genomic resources hinders its research at the molecular level. We have analyzed the S. trilobatum leaf transcriptome using high throughput RNA sequencing. The de novo assembly of 136,220,612 reads produced 128,934 non-redundant unigenes with N50 value of 1347 bp. Annotation of unigenes was performed against databases such as NCBI nr database,...
Published on Sep 1, 2018
T. Muralisankar (BDU: Bharathidasan University), P. Saravana Bhavan8
Estimated H-index: 8
(Bharathiar University)
+ 1 AuthorsP. Santhanam7
Estimated H-index: 7
(BDU: Bharathidasan University)
Experiments were conducted to evaluate the influence of dietary supplement (@ 5%) dried leaf powder of the medicinal herbs, Ocimum sanctum, Solanum trilobatum and Phyllanthus amarus on survival, growth, activities of digestive enzymes, energy utilization, and vitamin contents of the freshwater prawn, Macrobrachium rosenbergii. Results indicated significant (P < 0.05) improvements in survival, growth, activities of digestive enzymes, energy utilization, and vitamin levels in herb supplemented fee...
Published on Aug 22, 2017in Antioxidants
Kumar Ganesan4
Estimated H-index: 4
,
Kumeshini Sukalingam3
Estimated H-index: 3
,
Baojun Xu29
Estimated H-index: 29
Solanum trilobatum L. (Solanaceae) has been well known as nightshade, commonly used by diverse populations to heal several disorders. Earlier studies in Solanum trilobatum were focused on different pharmacological activities and a few were concerned with antioxidant and hepatoprotective effects. Thus, the current study was focused to evaluate the antioxidant potential and hepatoprotective effects of S. trilobatum L. on thioacetamide (TAA) intoxication in Wistar albino rats. The rats were kept in...
Published on Aug 1, 2017in Aquaculture Nutrition2.10
T. Muralisankar8
Estimated H-index: 8
(Bharathiar University),
P. Saravana Bhavan8
Estimated H-index: 8
(Bharathiar University)
+ 2 AuthorsRajarajeswaran Jayakumar11
Estimated H-index: 11
(UM: University of Malaya)
This study was conducted to evaluate the influence of herbs such as Ocimum sanctum, Phyllanthus amarus and Solanum trilobatum on the freshwater prawn, Macrobrachium rosenbergii, postlarvae (PL). These herbs were powdered and incorporated at 5% with the basal diet separately and in a combination. The M. rosenbergii was fed with these feeds for a period of 90 days. Results indicated that significant (P 0.05) improvement in survival and growth performance when compared to individual supplementation...
O estudo etnofarmacologico contribui para a descoberta de novos farmacos e de forma especial, aqueles voltados para a terapia anticancer. Os tumores acometem diversos tecidos de forma benigna ou maligna. Nesta ultima situacao inclui-se alem do crescimento anormal das celulas, a invasao de novos tecidos em virtude da dispersao das celulas tumorais, conduzindo o individuo a morte em muitos casos. Uma vez que os medicamentos anticancerigenos afetam gravemente as celulas normais, ressalta-se que o u...
Published on Mar 1, 2016in Toxins3.90
Szymon Chowański4
Estimated H-index: 4
(Adam Mickiewicz University in Poznań),
Zbigniew Adamski11
Estimated H-index: 11
(Adam Mickiewicz University in Poznań)
+ 8 AuthorsFilomena Lelario10
Estimated H-index: 10
(Adam Mickiewicz University in Poznań)
Only a small percentage of insect species are pests. However, pest species cause significant losses in agricultural and forest crops, and many are vectors of diseases. Currently, many scientists are focused on developing new tools to control insect populations, including secondary plant metabolites, e.g., alkaloids, glycoalkaloids, terpenoids, organic acids and alcohols, which show promise for use in plant protection. These compounds can affect insects at all levels of biological organization, b...
Published on Jan 1, 2016
The objective of the present study was to evaluate the anti-tumor activity of Leucopyrosinol isolated from the leaves parts of Fluggea leucopyrus. Antitumor activity of Fluggea leucopyrus against EAC was determined using single dose and intermittent treatments over two weeks, i.e. four-drug administration by the intrapretonial route on days, 1, 5, 9, and 11 after EAC cells inoculation. The compound and the vehicle were administered I.P using 10 mice per test group. Results indicated that there w...
View next paperA pilot study on the clinical efficacy of Solanum xanthocarpum and Solanum trilobatum in bronchial asthma.