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Identification of ZAG Protein as a Novel Serologic Biomarker Candidate for Liver Cancer

Published on Sep 1, 2011in Advanced Materials Research
· DOI :10.4028/www.scientific.net/AMR.340.383
Fei Wang3
Estimated H-index: 3
(Tianjin Medical University),
Yu Geng1
Estimated H-index: 1
+ 1 AuthorsXinGeng5
Estimated H-index: 5
(Tianjin Medical University)
Abstract
To identify Zinc-alpha-2-glycoprotein (ZAG) expression in HCC for serum biomarker, by analyzing the serum proteome of the patients suffering from primary hepatocellular carcinoma (HCC), liver cirrhosis and healthy donors. The serum proteome of the patients from HCC, liver cirrhosis and healthy donors were separated and identified by two-dimensional electrophoresis. The differentially expressed proteins were analyzed by peptide mass fingerprint based on MALDI-TOF-MS and SWISS-PROT or BLAST nr database searching. RT-PCR and Western blotting analysis were used to confirm expression of ZAG in HCC. Five differentially expressed proteins were identified. Albumin, Serotransferrin, CD5 antigen-like precursor ( IgM - associated peptide) were down-regulated in HCC, ZAG and Ig gamma-1 chain C region were up-regulated in HCC. ZAG, a lipid mobilizing factor, is a member of the major histocompatibility complex (MHC) class I family of protein. Five proteins which were found differentially expressed in HCC provided useful information for screening diagnostic tumor markers of human HCC. ZAG might be a novel candidate serum biomarker for HCC early diagnosis.
  • References (11)
  • Citations (3)
References11
Newest
#1Xin-Qing PengH-Index: 1
#2Fei Wang (Tianjin Medical University)H-Index: 3
Last.Wei-Ming Zhang (Tianjin Medical University)H-Index: 1
view all 4 authors...
#1Yueguo Li (Tianjin Medical University)H-Index: 1
#2Xin Geng (Tianjin Medical University)H-Index: 1
Last.Weiming Zhang (Tianjin Medical University)H-Index: 1
view all 3 authors...
#1Xiu-qin Xu (Genome Institute of Singapore)H-Index: 2
#2Chon K. Leow (Genome Institute of Singapore)H-Index: 1
Last.Hon-chiu Eastwood Leung (Genome Institute of Singapore)H-Index: 2
view all 9 authors...
#1ZhenCai (HKU: University of Hong Kong)H-Index: 4
#2Jen-FuChiu (HKU: University of Hong Kong)H-Index: 32
Last.Qing-YuHe (HKU: University of Hong Kong)H-Index: 35
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#1Laura F. Steel (Thomas Jefferson University)H-Index: 15
#2Donna Shumpert (Thomas Jefferson University)H-Index: 1
Last.Timothy M. Block (Thomas Jefferson University)H-Index: 55
view all 8 authors...
#1SeungOeLim (SNU: Seoul National University)H-Index: 22
#2Sung-Jun Park (SNU: Seoul National University)H-Index: 5
Last.Gu-HungJung (SNU: Seoul National University)H-Index: 27
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#1Terence C.W. Poon (CUHK: The Chinese University of Hong Kong)H-Index: 23
#2Philip J. Johnson (CUHK: The Chinese University of Hong Kong)H-Index: 80
Cited By3
Newest
#1Yanjie Zhao (CSU: Central South University)H-Index: 2
#2Qiang Ju (CSU: Central South University)H-Index: 3
Last.Guancheng Li (CSU: Central South University)H-Index: 14
view all 3 authors...
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