Direct penicillin G acylase immobilization by using the self-prepared immobilized metal affinity membrane

The use of penicillin G acylase (PGA) requires efficient methods for immobilizing the enzyme to yield a biocatalyst with high activity and stability for industrial application. This study constructs the immobilized metal affinity membrane (IMAM) via a series of chemical reactions to sequentially couple epichlorohydrin (EPI), iminodiacetic acid (IDA), and copper ions on the regenerated cellulose membrane. The feasibility of direct PGA...