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Demonstration of loss of attenuation and extended field persistence of a live avian metapneumovirus vaccine

Published on Oct 1, 2006in Vaccine3.27
· DOI :10.1016/j.vaccine.2006.06.076
E. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna),
Cecchinato Mattia6
Estimated H-index: 6
(UNIBO: University of Bologna)
+ 2 AuthorsC. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
Cite
Abstract
A live A type avian metapneumovirus (AMPV) vaccine which had been shown to be highly protective and short lived in experimental conditions was found to persist for longer periods in the field and to be associated with disease. Previously other factors such as possible secondary pathogens and management considerations had made it impossible to conclude whether the observed disease was a result of an increase in the vaccine virulence. In this study, an AMPV was isolated from poults on a farm which had been vaccinated with the same live A type vaccine. Full sequencing of the isolate, the vaccine and the vaccine progenitor confirmed its vaccine origin and further showed that generation of the vaccine had only involved nine substitutions of which three coded for amino acid changes. The isolated virus was inoculated into 1-day-old turkey poults in disease secure isolators and shown to cause disease with a severity similar to that caused by virulent field virus. Only two coding mutations were associated with this reversion to virulence.
  • References (33)
  • Citations (59)
Cite
References33
Newest
Published on Aug 1, 2005in Avian Pathology1.96
Kannan Ganapathy12
Estimated H-index: 12
(University of Liverpool),
P. Cargill4
Estimated H-index: 4
(Merial)
+ 1 AuthorsR. C. Jones35
Estimated H-index: 35
(University of Liverpool)
One-day-old specific pathogen free White Leghorn chicks were vaccinated with live avian pneumovirus (APV) vaccine, live Newcastle disease virus (NDV) vaccine or both. At intervals up to 28 days after vaccination, distribution of the virus in the tissues was studied, together with humoral and mucosal antibody responses in lachrymal fluid and tracheal washes. APV vaccine was detected for almost twice as long in the dual vaccinates as in the single vaccinates. Higher numbers of isolations of ND vir...
Published on Jun 1, 2005in Avian Pathology1.96
Caroline Banet-Noach14
Estimated H-index: 14
,
Lubov Simanov9
Estimated H-index: 9
,
Shimon Perk9
Estimated H-index: 9
Although the disease associated with the avian metapneumovirus (aMPV) has been described in Israel for more than 20 years and one Israeli isolate was characterized as subtype A, this is the first study investigating the strains circulating in Israel (period 2002 to 2004). Viral RNA was detected by reverse transcription-polymerase chain reaction in 44% of the examined flocks, and one-half of the virus isolation attempts were successful. Characterization showed that 34 non-vaccinated turkey flocks...
Published on Jan 1, 2005in New Microbiologica1.59
Emanuela Foni18
Estimated H-index: 18
,
Chiara Chiapponi19
Estimated H-index: 19
+ 4 AuthorsP. Massi2
Estimated H-index: 2
Cloacal swabs collected from mallard ducks (Anas platyrhynchos) experimentally infected with a H7N1 avian influenza strain were examined by Reverse Transcription Polymerase Chain Reaction to detect the influenza A virus. Reverse Transcription Polymerase Chain Reaction was compared with standard methods: inoculation of embryonated chicken eggs and inoculation of three established cell lines: Newborn Swine Kidney cells, Newborn Pig Trachea cells and Madine Darby Canine Kidney cells. Reverse Transc...
Published on Nov 1, 2004in Journal of General Virology2.81
C. J. Naylor17
Estimated H-index: 17
(University of Liverpool),
Paul A. Brown4
Estimated H-index: 4
(University of Liverpool)
+ 4 AuthorsAndrew J. Easton37
Estimated H-index: 37
(Warw.: University of Warwick)
Avian pneumovirus (APV) is a member of the genus Metapneumovirus of the subfamily Pneumovirinae. This study describes the development of a reverse-genetics system for APV. A minigenome system was used to optimize the expression of the nucleoprotein, phosphoprotein, M2 and large polymerase proteins when transfected into Vero cells under the control of the bacteriophage T7 promoter. Subsequently, cDNA was transcribed from the virion RNA to make a full-length antigenome, which was also cloned under...
Published on Apr 1, 2004in Avian Diseases1.31
Darrell R. Kapczynski26
Estimated H-index: 26
Abstract An avian metapneumovirus (aMPV) virosome vaccine was prepared and tested for protection of turkeys by aMPV challenge. The vaccine was produced using a detergent-based (Triton X-100) extraction of aMPV subtype C followed by detergent removal with SM2 Bio-Beads. Western blot and virus-neutralization analysis confirmed that the aMPV virosomes contained both the fusion and attachment glycoproteins. Specific-pathogen-free turkeys were immunized either intranasally (IN) or intramuscularly (IM...
Published on Oct 1, 2003in Avian Diseases1.31
Darrell R. Kapczynski26
Estimated H-index: 26
(USDA: United States Department of Agriculture),
Holly S. Sellers16
Estimated H-index: 16
(USDA: United States Department of Agriculture)
SUMMARY. In this study we compared protection by DNA vaccination with the F (pCMVF) or N (pCMV-N) gene from avian metapneumovirus (aMPV) in turkeys. One-week-old turkey poults received two intramuscular injections 2 wk apart. Birds were challenged with a turkey-embryo-adapted aMPV at 5 wk of age. Birds vaccinated with pCMV-F had decreased clinical signs of disease as well as significantly reduced virus load in tracheal swabs compared with birds vaccinated with pCMV-N or unvaccinated control bird...
Published on Aug 1, 2001in Veterinary Microbiology2.79
S. Van de Zande4
Estimated H-index: 4
(UGent: Ghent University),
Hans Nauwynck4
Estimated H-index: 4
(UGent: Ghent University),
Maurice Pensaert45
Estimated H-index: 45
(UGent: Ghent University)
Abstract The purpose of this study was to evaluate the effect of an Escherichia coli infection in avian pneumovirus (APV)-infected turkeys. One group of 2-week-old specific pathogen-free (SPF) and two groups of 3-week-old conventional (CON) turkeys were inoculated oculonasally with virulent APV subtype A alone, with E. coli O2:K1 alone or with both agents at varying intervals (1, 3, 5 or 7 days) between the two inoculations. The birds were followed clinically and examined for macroscopic lesions...
Published on Apr 1, 2001in Avian Pathology1.96
E. W. Aldous1
Estimated H-index: 1
,
D. J. Alexander1
Estimated H-index: 1
Substantial variation in the virulence of Newcastle disease virus (NDV) isolates means that the detection of NDV or evidence of infection is insufficient for an adequate diagnosis, as control measures for avirulent viruses are very different to those for virulent viruses. Diagnosis therefore requires further characterization, at least as to whether an isolate is virulent or avirulent. Conventional detection and differentiation of ND viruses is perceived as slow, laborious and requiring an undesi...
Published on Nov 1, 2000in Journal of General Virology2.81
M. H. Bäyon-Auboyer5
Estimated H-index: 5
,
Claire Arnauld3
Estimated H-index: 3
+ 1 AuthorsN. Eterradossi23
Estimated H-index: 23
Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99·7% nt and 99·0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70·0–80·5% and 77·6–97·2%, respectively, with the L gene sharing 76·1% nt and 85·3% aa identity with that of a...
Published on Jul 29, 2000in Veterinary Record2.10
S. Van de Zande4
Estimated H-index: 4
(UGent: Ghent University),
Hans Nauwynck4
Estimated H-index: 4
(UGent: Ghent University)
+ 1 AuthorsC. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
The degree and duration of clinical and virological cross-protection between avian pneumovirus subtypes A and B were examined in two-week-old pneumovirus antibody-free turkeys. The turkeys were inoculated with either a virulent subtype A (Belgian isolate AIT6/96), a virulent subtype B (Belgian isolate B/T9/96), an attenuated subtype A or an attenuated subtype B, and challenged homologously and heterologously with virulent avian pneumovirus two, five and 11 weeks after inoculation. Birds inoculat...
Cited By59
Newest
Published on May 1, 2019in Vaccine3.27
Andrea Laconi3
Estimated H-index: 3
(University of Liverpool),
E. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
+ 1 AuthorsC. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
Abstract Since late ‘80 s Avian metapneumovirus subtype A causes sufficient disease in Europe for commercial companies to have started developing live attenuated vaccines. Here, two of those vaccines were fully consensus sequenced alongside their progenitor field strain (#8544). Sequences comparison shows that the attenuation of field strain #8544 was associated with no common substitutions between the two derived vaccines. This finding suggests that the attenuation of field viruses via serial p...
Published on Feb 1, 2018in Poultry Science2.03
Claudia Maria Tucciarone5
Estimated H-index: 5
(UNIPD: University of Padua),
Giovanni Franzo10
Estimated H-index: 10
(UNIPD: University of Padua)
+ 7 AuthorsMattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
Published on Dec 1, 2017in Scientific Reports4.01
Haixia Hu3
Estimated H-index: 3
(SWU: Southwest University),
Jason P. Roth3
Estimated H-index: 3
(Merial)
+ 1 AuthorsQingzhong Yu13
Estimated H-index: 13
(USDA: United States Department of Agriculture)
Avian metapneumovirus (AMPV) infects the respiratory and reproductive tracts of domestic poultry, resulting in substantial economic losses for producers. Live attenuated vaccines appear to be the most effective in countries where the disease is prevalent. However, reversion to virulence has been demonstrated in several studies. Therefore, the development of a stable and safe next generation vaccine against the AMPV disease is needed. In the present study, we generated a recombinant Newcastle dis...
Published on Oct 1, 2017in Vaccine3.27
V. Listorti4
Estimated H-index: 4
(University of Liverpool),
Andrea Laconi3
Estimated H-index: 3
(University of Liverpool)
+ 3 AuthorsC. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
Abstract IBV genotype QX causes sufficient disease in Europe for several commercial companies to have started developing live attenuated vaccines. Here, one of those vaccines (L1148) was fully consensus sequenced alongside its progenitor field strain (1148-A) to determine vaccine markers, thereby enabling detection on farms. Twenty-eight single nucleotide substitutions were associated with the 1148-A attenuation, of which any combination can identify vaccine L1148 in the field. Sixteen substitut...
Published on Sep 1, 2017in Avian Diseases1.31
Claudia Maria Tucciarone5
Estimated H-index: 5
,
Marianna Andreopoulou + 3 AuthorsMattia Cecchinato12
Estimated H-index: 12
SUMMARY Avian metapneumovirus (aMPV) is considered a major pathogen for turkeys but its impact on chicken production is still partially neglected, even though it is fully acknowledged as a primary pathogen in chickens as well. The lack of structured diagnostic surveys does not allow a pervasive understanding of aMPV epidemiology. Being that aMPV is almost an everyday challenge for farmers and veterinarians, a more accurate report of its presence should be detailed, posing the basis for a deep an...
Published on Dec 15, 2016in Journal of Virology4.32
Bingling Yun6
Estimated H-index: 6
(Harbin Veterinary Research Institute),
Yao Zhang3
Estimated H-index: 3
(Harbin Veterinary Research Institute)
+ 11 AuthorsHonglei Gao19
Estimated H-index: 19
(Harbin Veterinary Research Institute)
Published on Jul 3, 2016in Avian Pathology1.96
C. Lupini3
Estimated H-index: 3
(UNIBO: University of Bologna),
Davide Giovanardi7
Estimated H-index: 7
+ 6 AuthorsE. Catelli3
Estimated H-index: 3
(UNIBO: University of Bologna)
ABSTRACTA distinctive infectious bursal disease (IBD) virus genotype (ITA) was detected in IBD-live vaccinated broilers in Italy without clinical signs of IBD. It was isolated in specific-pathogen-free eggs and molecularly characterized in the hypervariable region of the virus protein (VP) 2. Phylogenetic analysis showed that ITA strains clustered separately from other homologous reference sequences of IBDVs, either classical or very virulent, retrieved from GenBank or previously reported in Ita...
Published on Sep 1, 2015in Brazilian Journal of Poultry Science0.61
Luciana Konecny Kohn12
Estimated H-index: 12
(State University of Campinas),
Mary Ann Foglio22
Estimated H-index: 22
(State University of Campinas)
+ 5 AuthorsClarice Weiss Arns4
Estimated H-index: 4
(State University of Campinas)
Avian metapneumovirus (aMPV) is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to theParamyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determi...
Published on May 1, 2015in Vaccine3.27
Giovanni Franzo10
Estimated H-index: 10
(UNIPD: University of Padua),
C. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
+ 5 AuthorsMattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
Abstract Avian metapneumovirus (aMPV) infects respiratory and reproductive tracts of domestic poultry, often involving secondary infections, and leads to serious economic losses in most parts of the world. While in general disease is effectively controlled by live vaccines, reversion to virulence of those vaccines has been demonstrated on several occasions. Consensus sequence mutations involved in the process have been identified in more than one instance. In one previous subtype A aMPV candidat...
Published on Jan 1, 2015
Abdel-Satar Arafa13
Estimated H-index: 13
,
Wessam Mady1
Estimated H-index: 1
+ 2 AuthorsHanafy M. Madbouly2
Estimated H-index: 2
Avian metapneumovirus (aMPV) causes acute respiratory infection in turkeys with sudden onset and rapid spread in the infected flocks. Vaccinated flocks frequently showed clinical signs typical like the non-vaccinated flocks in Egypt, this situation suggests a virulent vaccine virus was circulating in the field. Oropharyngeal and nasal sinus swabs were collected from 100 turkeys from 10 commercial farms with mild to moderate respiratory signs. Virus isolation and propagation was done in SPF embry...
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