The impact of Lys→Arg surface mutations on the crystallization of the globular domain of RhoGDI

The potential of rational surface mutagenesis for enhanced protein crystallization is being probed in an ongoing effort. In previous work, it was hypothesized that residues with high conformational entropy such as Glu and Lys are suitable targets for surface mutagenesis, as they are rarely incorporated in crystal contacts or protein–protein interfaces. Previous experiments using Lys→Ala, Glu→Ala and Glu→Asp mutants confirmed that mutated...