Match!

Epimedium‐Derived Phytoestrogen Flavonoids Exert Beneficial Effect on Preventing Bone Loss in Late Postmenopausal Women: A 24‐Month Randomized, Double‐Blind and Placebo‐Controlled Trial

Published on Apr 9, 2007in Journal of Bone and Mineral Research5.711
· DOI :10.1359/jbmr.070405
Ge Zhang32
Estimated H-index: 32
(SHU: Shanghai University),
Ling Qin53
Estimated H-index: 53
(CUHK: The Chinese University of Hong Kong),
Yinyu Shi4
Estimated H-index: 4
(SHU: Shanghai University)
Sources
Abstract
Epimedium brevicornum maxim, a nonleguminous medicinal plant, has been found to be rich in phytoestrogen flavonoids. Results from a 24-month randomized double-blind placebo-controlled clinical trial showed that Epimedium-derived phytoestrogen flavonoids were able to exert beneficial effects on preventing bone loss in late postmenopausal women, without resulting in a detectable hyperplasia effect on the endometrium. Introduction: We performed a 24-mo randomized double-blind placebo-controlled clinical trial for evaluating the effect of the Epimedium-derived phytoestrogen flavonoids (EPFs) on BMD, bone turnover biochemical markers, serum estradiol, and endometrial thickness in postmenopausal women. Materials and Methods: One hundred healthy late postmenopausal women, with a natural menopausal history within 10 ∼18 yr and with a BMD T-score at the lumbar spine between −2 and −2.5 SD, were randomized into EPF treatment group (n = 50; a daily dose of 60 mg Icariin, 15 mg Daidzein, and 3 mg Genistein) or placebo control group (n = 50). All participants received 300 mg element calcium daily. BMD, bone turnover biochemical markers, serum estradiol, and endometrial thickness were measured at baseline and 12 and 24 mo after intervention. Results: Eighty-five participants completed the trial. The patterns of BMD changes were significantly different between the EPF treatment group and placebo control group by repeated-measures ANOVA (p = 0.045 for interaction between time and group at femoral neck; p = 0.006 for interaction between time and group at lumbar spine). BMD was found with a decreased tendency in the placebo control group at 12 (femoral neck: −1.4%, p = 0.104; lumbar spine: −1.7%, p = 0.019) and 24 mo (femoral neck: −1.8%, p = 0.048; lumbar spine: −2.4%, p = 0.002), whereas EPF treatment maintained BMD at 12 (femoral neck: 1.1%, p = 0.285; lumbar spine:1.0%, p = 0.158) and 24 mo (femoral neck: 1.6%, p = 0.148; lumbar spine: 1.3%, p = 0.091). The difference in lumbar spine between the two groups was significant at both 12 (p = 0.044) and 24 mo (p = 0.006), whereas the difference in the femoral neck was marginal at 12 mo (p = 0.061) and significant at 24 mo (p = 0.008). Levels of bone biochemical markers did not change in the placebo control group. In contrast, EPF intervention significantly decreased levels of deoxypyrdinoline at 12 (−43%, p = 0.000) and 24 mo (−39%, p = 0.000), except for osteocalcin at 12 (5.6%, p = 0.530) and 24 mo (10.7%, p = 0.267). A significant difference in deoxypyrdinoline between the two groups was found at both 12 (p = 0.000) and 24 mo (p = 0.001). Furthermore, neither serum estradiol nor endometrial thickness was found to be changed in either groups during the clinical trial. Conclusions: EPFs exert a beneficial effect on preventing bone loss in late postmenopausal women without resulting in a detectable hyperplasia effect on the endometrium.
  • References (63)
  • Citations (153)
📖 Papers frequently viewed together
4 Authors (Hua Nian, ..., L.-L. Xu)
129 Citations
88 Citations
106 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References63
Newest
#1Hui Ying Huang (TMU: Taipei Medical University)H-Index: 15
#2Hsiao-Ping Yang (TMUH: Taipei Medical University Hospital)H-Index: 1
Last. Shih Yi Huang (TMU: Taipei Medical University)H-Index: 24
view all 6 authors...
Abstract It is believed that soy isoflavone has much potential effectiveness on the postmenopausal status; however, the optimal dose for preventing postmenopausal bone loss still remains unclear. This open-labeled, self-controlled pilot study was undertaken to determine the effect of 1-year supplementation of different high dosages of soy isoflavone in postmenopausal Taiwanese women. Forty-three women aged 45–67 years were enrolled and randomly assigned into a control (C), 100 mg/day isoflavone ...
38 CitationsSource
#1Ge Zhang (CUHK: The Chinese University of Hong Kong)H-Index: 32
#2Ling Qin (CUHK: The Chinese University of Hong Kong)H-Index: 53
Last. Kwong-Sak Leung (CUHK: The Chinese University of Hong Kong)H-Index: 40
view all 7 authors...
Aim: Factorial design was used to test our hypothesis whether a group of flavonoids (FE) derived from herbal Epimedium Brevicornum Maxim exerted its preventive effects on estrogen-deficiency-induced osteoporosis mainly through an enhancement in intestinal calcium absorption. Materials and methods: Forty-five 12-month-old female Wistar rats were used and randomly assigned into sham-operated group and four ovariectomy (OVX) subgroups, i.e. OVX with vehicle (OVX group), OVX with FE (FE group), OVX ...
94 CitationsSource
High consumption of phytoestrogen-rich food correlates with reduced incidence of breast cancer. However, the effect of phytoestrogens on growth of pre-existing breast tumors presents concerns when planning the use of phytoestrogens as chemoprevention strategy. Genistein, the active phytoestrogen in soy, displays weak estrogenic activity mediated by estrogen receptor (ER) with a preferential binding for the ER-B species. However, no information is at present available on the interaction between p...
35 CitationsSource
#1Zuzana Mackova (Institute of Chemical Technology in Prague)H-Index: 4
#2Radka Koblovská (Institute of Chemical Technology in Prague)H-Index: 6
Last. Oldrich Lapcik (Institute of Chemical Technology in Prague)H-Index: 4
view all 3 authors...
Abstract Common emphasis of the fact that isoflavonoids are characteristic metabolites of leguminous plants sometimes leads to overlooking that the presence of isoflavonoids has been reported in several dozen other families. The spectrum of isoflavonoid producing taxa includes the representatives of four classes of multicellular plants, namely the Bryopsida, the Pinopsida, the Magnoliopsida and the Liliopsida. A review, recently published by Reynaud et al. [Reynaud, J., Guilet D., Terreux R., Lu...
37 CitationsSource
#1Jian WuH-Index: 38
#2Jun OkaH-Index: 19
Last. Yoshiko IshimiH-Index: 23
view all 11 authors...
The combined intervention of isoflavone intake and walking exercise over 1 year in postmenopausal Japanese women exhibited a trend for a greater effect on prevention of bone loss at the total hip and Ward's triangle regions. Introduction: The additive effects of isoflavones and exercise on bone and lipid metabolism have been shown in estrogen-deficient animals. In this study, we determined the effects of isoflavone intake, walking exercise, and their interaction on bone, fat mass, and lipid meta...
94 CitationsSource
Interest in the physiologic and pharmacologic role of bioactive compounds present in plants has increased dramatically over the last decade. Of particular interest in relation to human health are the classes of compounds known as the phytoestrogens, which embody several groups of non-steroidal estrogens, including isoflavones and lignans that are widely distributed within nature. The impact of dietary phytoestrogens on normal biologic processes was first recognized in sheep. Observations of shee...
174 CitationsSource
#1Chen KmH-Index: 3
#2Ge BfH-Index: 3
Last. Wang YH-Index: 3
view all 6 authors...
The herb Epimedium has long been used in Traditional Chinese Medicine to treat bone fracture and prevent osteoporosis. Researchers believe that the flavonoids contained in the herb are the effective component for this activity. However, no single flavonoid has been studied for its effect on bone-related cells. In the present study, icariin, one of the major flavonoids of the herb, supplemented the primary culture medium of rat bone marrow stromal cells (rMSCs) at 0.1 μM, 1 μM and 10 μM respectiv...
67 Citations
#1Pierangela TottaH-Index: 15
#2Filippo AcconciaH-Index: 29
Last. Maria MarinoH-Index: 40
view all 7 authors...
Daidzein (D), a soy isoflavone, is almost completely metabolized in the gut and liver. This biotransformation converts D to more water-soluble products and may affect its biological activity. The ability of daidzein metabolites to modulate 17/3-estradiol (E2)-sensitive gene transcription, cell growth, and a proapoptotic cascade was determined in human cancer cells devoid of any estrogen receptor (ER) and rendered E2 sensitive after transfection with ER/3. The data show that D and some but not al...
59 CitationsSource
#1Xiao Qiangbing (HUST: Huazhong University of Science and Technology)H-Index: 1
#2Chen Anmin (HUST: Huazhong University of Science and Technology)H-Index: 4
Last. Guo Fengjin (HUST: Huazhong University of Science and Technology)H-Index: 1
view all 3 authors...
To study the effects of Icariin on expression of osteopontin (OPN) mRNA and type I collagen in rat osteoblastsin vitro and to explore its possible mechanisms in preventing osteoporosis. OB was isolated from calvaria of new-born new-born fetal Sprague-Dawley (SD) rats by means of modified sequential collagenase digestion and incubated in MEM medium and the cell morphology was observed under inverted phase contrast microscope, OB was identified by alkaline phosphatase (ALP) staining. Different con...
24 CitationsSource
#1Konrad F. Koehler (Novum)H-Index: 22
#2Luisa A. Helguero (KI: Karolinska Institutet)H-Index: 23
Last. Jan-Åke Gustafsson (KI: Karolinska Institutet)H-Index: 146
view all 5 authors...
We have known for many years that estrogen is more than the female hormone. It is essential in the male gonads, and in both sexes, estrogen has functions in the skeleton and central nervous system, on behavior, and in the cardiovascular and immune systems. An important aspect of the discovery of estrogen receptor (ER) β is that the diverse functions of estrogen can now be divided into those mediated by ERα and those mediated by ERβ. Pharmacological exploitation of this division of the labors of ...
307 CitationsSource
Cited By153
Newest
Abstract Background/objectives For treatment of large bone defects challenging in orthopaedic clinics, bone graft substitutes are commonly used for the majority of surgeons. It would be proposed in the current study that our bioactive scaffolds could additionally serve as a local delivery system for therapeutic small molecule agents capable of providing support to enhance biological bone repair. Methods In this study, composite scaffolds made of poly (lactic-co-glycolic acid) (PLGA) and tricalci...
Source
#1Zuoying Yuan (PKU: Peking University)
#2Zhuo Wan (Huada: Beijing University of Chemical Technology)
Last. Xiaoping Yang (Huada: Beijing University of Chemical Technology)H-Index: 34
view all 8 authors...
Current scaffolds applied for bone tissue engineering are still lacking sufficient osteogenic capacity to induce efficient bone regeneration. Biodegradable microsphere-type scaffolds are designed to achieve the dual-controlled release of a Chinese medicine (i.e., icariin, ICA) and a bioactive ion (i.e., Mg(2+) ), in order to achieve their synergistic effect on inducing osteogenesis. The hydrophobic icariin is preloaded onto MgO/MgCO3 (1:1 in weight ratio) particles at different amounts and then ...
Source
#1Jing Cui (Zhengzhou University)
#2Hao Li (Zhengzhou University)
Last. Baisui Feng (Zhengzhou University)
view all 8 authors...
Baicalein has been widely studied and showed a potent activity against pancreatic cancer in both in vivo and in vitro studies. Little is known regarding the effects of Skullcapflavone I (SFI), despite they have similar structures. So, this study was to explore the function of SFI on human pancreatic cancer. Panc-1 cells were transfected with miR-23a precursor, miR-23a inhibitor or the negative controls, and subsequently treated by SFI. Cell viability, Bromodeoxyuridine (BrdU)-positive cell rate,...
Source
#1Li-Ping Zhou (PolyU: Hong Kong Polytechnic University)H-Index: 4
#2Christina C.-W. Poon (PolyU: Hong Kong Polytechnic University)H-Index: 1
Last. Man-Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 9 authors...
Icariin, a flavonoid phytoestrogen derived from Herba epimedii, has been reported to exert estrogenic effects in bone and activate phosphorylation of estrogen receptor (ER) alpha in osteoblastic cells. However, it is unclear whether icariin selectively exerts estrogenic activities in bone without inducing undesirable effects in other estrogen-sensitive tissues. The present study aimed to investigate the tissue-selective estrogenic activities of icariin in estrogen-sensitive tissues in vivo and i...
Source
#1Xi-yang Tang (MOE: Chinese Ministry of Education)
#2Zi-qin Dai (MOE: Chinese Ministry of Education)
Last. Xin-sheng Yao (MOE: Chinese Ministry of Education)
view all 9 authors...
Abstract Xian-Ling-Gu-Bao capsule (XLGB) is an effective traditional Chinese medicine prescription (TCMP) that is used for the prevention and treatment of osteoporosis in China. A rapid, simple, efficient and stable method based on UPLC-MS/MS technology was developed for simultaneous determination of multiple components of XLGB in rat plasma. Mass spectrometric detection was performed in multiple reaction monitoring (MRM) mode with electrospray ionization (ESI). For twenty-one selected quantitat...
1 CitationsSource
#1Yu-Hao ChengH-Index: 1
#2Jingcheng DongH-Index: 1
Last. Qin BianH-Index: 12
view all 3 authors...
Mesenchymal stem cells (MSCs) are adult stem cells harboring self-renewal and multilineage differentiation potential that are capable of differentiating into osteoblasts, adipocytes, or chondrocytes in vitro, and regulating the bone marrow microenvironment and adipose tissue remodeling in vivo. The process of fate determination is initiated by signaling molecules that drive MSCs into a specific lineage. Impairment of MSC fate determination leads to different bone and adipose tissue-related disea...
1 CitationsSource
#1Yuexin Xu (Nanjing Medical University)H-Index: 3
#2Lingyun Li (Nanjing Medical University)H-Index: 1
Last. Changyan Ma (Nanjing Medical University)H-Index: 5
view all 6 authors...
Abstract Osteoporosis is a bone disease characterized by microarchitectural deterioration, low bone mass, and increased risk of fractures. Icariin (ICA), an active flavonoid glucoside isolated from Herba epimedii (HEF), is a potent stimulator of osteogenic differentiation and has potential applications for preventing bone loss in postmenopausal women. However, the molecular mechanism underlying the osteogenic effect of ICA has not yet been fully elucidated. In this study, we report that ICA trea...
Source
#1Man-Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 33
#2Christina C.-W. Poon (PolyU: Hong Kong Polytechnic University)H-Index: 1
Last. Hui-Hui Xiao (PolyU: Hong Kong Polytechnic University)H-Index: 7
view all 4 authors...
Demands for natural products, in the form of botanicals, dietary supplements, and herbal medicine, for management of chronic diseases are increasing globally. Natural products might be an alternative for the management of bone health to meet the demands of a growing aging population. Different types of natural products, including Chinese herbal medicine decoctions, herbs, and isolated phytochemicals, have been demonstrated to exert bone protective effects. The most common types of bone protectiv...
Source
#1Jian-bo HeH-Index: 2
#1Jianbo He (Guangzhou University of Chinese Medicine)H-Index: 2
Last. Dingkun LinH-Index: 3
view all 13 authors...
Abstract: Osteoporosis is a bone disease characterized by increasing osseous fragility and fracture due to the reduced bone mass and microstructural degradation. Primary pharmacological strategies for the treatment of osteoporosis, hormone replacement (HRT) and alendronate therapies, may produce adverse side-effects and may not be recommended for long-term usage. Complementary and alternative medicines may provide effective treatment for osteoporosis without the risk of adverse side-effects. Cur...
Source
Osteoporosis is rapidly turning into a global epidemic, leading to high rates of morbidity and mortality due to the resulting complications such as osteoporotic fractures in older people. Therefore, this review investigated the findings of clinical trials regarding the effects of medicinal herbs on osteoporosis. To this end, several keywords were used to search for relevant articles indexed in the Institute for Scientific Information and PubMed, including "Osteoporosis" or "Bone loss" AND "medic...
Source