Dependence of lysozyme growth kinetics on step sources and impurities

Interferometric microscopy was used to investigate the growth morphology and kinetics of {110} and {101} faces of tetragonal lysozyme crystals. Solutions were prepared from as-received Sigma and Seikagaku material, and Seikagaku lysozyme further purified by cation exchange liquid chromatography under salt-free conditions. The protein composition of the solutions was characterized by sodium dodecyl sulphate (SDS) electrophoresis with silver...