Functional Consequences of Mutations in the Human α_1ACalcium Channel Subunit Linked to Familial Hemiplegic Migraine

Mutations in α 1A , the pore-forming subunit of P/Q-type calcium channels, are linked to several human diseases, including familial hemiplegic migraine (FHM). We introduced the four missense mutations linked to FHM into human α 1A-2 subunits and investigated their functional consequences after expression in human embryonic kidney 293 cells. By combining single-channel and whole-cell patch-clamp recordings, we show that all four mutations affect...