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Molecular properties and antioxidant activities of polysaccharides isolated from alkaline extract of wild Armillaria ostoyae mushrooms

Published on Feb 1, 2016in Carbohydrate Polymers6.044
· DOI :10.1016/j.carbpol.2015.05.061
Ka-Chai Siu8
Estimated H-index: 8
(PolyU: Hong Kong Polytechnic University),
Li-Jian Xu4
Estimated H-index: 4
(Heilongjiang University)
+ 1 AuthorsJian-Yong Wu48
Estimated H-index: 48
(PolyU: Hong Kong Polytechnic University)
Sources
Abstract
Abstract This study aims to discover novel and bioactive polysaccharides (PS) from wild Armillaria ostoyae , a honey mushroom species. Two PS designated AkPS1V-1 (66.6 kDa) and AkPS1V-2 (15.3 kDa) were isolated and fractionated by anion ion exchange (IEC) and size exclusion chromatography (SEC) from the alkaline extract of A. ostoyae mushrooms. AkPS1V-1 was a glucan composed of solely glucose residues and AkPS1V-2 a heteropolysaccharide composed of glucose and galactose at 6:1 molar ratio. AkPS1V-2 exhibited higher antioxidant activities than AkPS1V-1 based on reducing power, radical scavenging and metal chelating assays. The structure of AkPS1V-2 was further analyzed and elucidated as a branched galactoglucan with a backbone composed of (1→6)-β- d -glucopyranosyl, (1→3)-β- d -glucopyranosyl, (1→3)-α- d -galactopyranosyl and (1→3,6)-β- d -glucopyranosyl residues at 3:1:1:1 ratio, and side chain of (1→3)-β- d -glucopyranosyl residue. This is the first report on a pure PS structure and its antioxidant activities from this mushroom species.
  • References (33)
  • Citations (37)
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Abstract The major chemical constituents and antioxidant activities of water-soluble polysaccharide (PS) fractions isolated from three edible mushrooms were investigated. Crude PS (CPS) isolated from the mushroom hot water extract by ethanol precipitation contained 15–30% (w/w) total protein and 2–5% phenolics. The CPS were fractionated by ion exchange chromatography and the neutral, and slightly ionic PS fractions (eluted with 0 and 0.1 M NaCl) contained 70–80% carbohydrate with low protein and...
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