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Molecular properties and antioxidant activities of polysaccharides isolated from alkaline extract of wild Armillaria ostoyae mushrooms

Published on Feb 1, 2016in Carbohydrate Polymers6.04
· DOI :10.1016/j.carbpol.2015.05.061
Ka-Chai Siu8
Estimated H-index: 8
(PolyU: Hong Kong Polytechnic University),
Lijian Xu4
Estimated H-index: 4
(Heilongjiang University)
+ 1 AuthorsJian-Yong Wu44
Estimated H-index: 44
(PolyU: Hong Kong Polytechnic University)
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Abstract
Abstract This study aims to discover novel and bioactive polysaccharides (PS) from wild Armillaria ostoyae , a honey mushroom species. Two PS designated AkPS1V-1 (66.6 kDa) and AkPS1V-2 (15.3 kDa) were isolated and fractionated by anion ion exchange (IEC) and size exclusion chromatography (SEC) from the alkaline extract of A. ostoyae mushrooms. AkPS1V-1 was a glucan composed of solely glucose residues and AkPS1V-2 a heteropolysaccharide composed of glucose and galactose at 6:1 molar ratio. AkPS1V-2 exhibited higher antioxidant activities than AkPS1V-1 based on reducing power, radical scavenging and metal chelating assays. The structure of AkPS1V-2 was further analyzed and elucidated as a branched galactoglucan with a backbone composed of (1→6)-β- d -glucopyranosyl, (1→3)-β- d -glucopyranosyl, (1→3)-α- d -galactopyranosyl and (1→3,6)-β- d -glucopyranosyl residues at 3:1:1:1 ratio, and side chain of (1→3)-β- d -glucopyranosyl residue. This is the first report on a pure PS structure and its antioxidant activities from this mushroom species.
  • References (33)
  • Citations (33)
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References33
Newest
Published on Nov 1, 2014in Journal of Functional Foods3.20
Ka-Chai Siu8
Estimated H-index: 8
(PolyU: Hong Kong Polytechnic University),
Xia Chen7
Estimated H-index: 7
(PolyU: Hong Kong Polytechnic University),
Jian-Yong Wu44
Estimated H-index: 44
(PolyU: Hong Kong Polytechnic University)
Abstract The major chemical constituents and antioxidant activities of water-soluble polysaccharide (PS) fractions isolated from three edible mushrooms were investigated. Crude PS (CPS) isolated from the mushroom hot water extract by ethanol precipitation contained 15–30% (w/w) total protein and 2–5% phenolics. The CPS were fractionated by ion exchange chromatography and the neutral, and slightly ionic PS fractions (eluted with 0 and 0.1 M NaCl) contained 70–80% carbohydrate with low protein and...
Published on Feb 1, 2013in Process Biochemistry2.88
Qilin Huang3
Estimated H-index: 3
(PolyU: Hong Kong Polytechnic University),
Ka-Chai Siu8
Estimated H-index: 8
(PolyU: Hong Kong Polytechnic University)
+ 2 AuthorsJian-Yong Wu44
Estimated H-index: 44
(PolyU: Hong Kong Polytechnic University)
Abstract Exopolysaccharide (EPS) fractions P 1/5 , P 2/5 , P 1 , P 2 and P 5 were isolated from the fermentation medium of a medicinal fungus Cordyceps sinensis by gradient precipitation with ethanol at 1/5, 2/5, 1, 2, and 5 volume ratios to the liquid medium. P 1/5 and P 2/5 were mainly composed of polysaccharides with negligible protein content and a large molecular or particle size (intrinsic viscosity [ η ] 2025 mL/g; hydrodynamic radius R h 905 nm). The fraction attained at a higher ethanol...
Published on Nov 1, 2012in International Journal of Biological Macromolecules4.78
Jun Wu7
Estimated H-index: 7
(HMU: Harbin Medical University),
Jinxu Zhou2
Estimated H-index: 2
(HIT: Harbin Institute of Technology)
+ 4 AuthorsShidong Xu5
Estimated H-index: 5
(HMU: Harbin Medical University)
Abstract Armillaria mellea is a famous traditional Chinese medicinal and edible fungus. In this study, we purified a water-soluble polysaccharide (AMP) from the fruiting bodies of this fungus. AMP contained 94.8% carbohydrate, 2.3% uronic acid and 0.5% protein. Its molecular weight was determined as 4.6 × 10 5 Da, as determined by high-performance gel-permeation chromatography (HPGPC). Gas chromatography (GC) analysis indicated that AMP was mainly composed of d -glucose. In vitro assay, AMP exhi...
Published on May 1, 2012in Journal of Food Composition and Analysis2.99
Maja Kozarski10
Estimated H-index: 10
(University of Belgrade),
Anita Klaus11
Estimated H-index: 11
(University of Belgrade)
+ 4 AuthorsLeo J.L.D. Van Griensven25
Estimated H-index: 25
(WUR: Wageningen University and Research Centre)
Abstract Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum , Ganoderma lucidum , Lentinus edodes and Trametes versicolor , were studied. G. applanatum and L. edodes extracts were the strongest DPPH scavengers, i.e. EC 50 G. applanatum extract had the highest chelating ability of ferrous ion (EC 50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC 50 = 2.07 mg/...
Published on Apr 1, 2012in Journal of Food Biochemistry1.36
Li Wen Gao3
Estimated H-index: 3
(Soochow University (Suzhou)),
Jian Wen Wang19
Estimated H-index: 19
(Soochow University (Suzhou))
In this study, the antioxidant activities of hot-water extract from cultured mycelia of Armillaria mellea were assessed in different in vitro systems. The extract showed strongly inhibitory effect on microsomal liposome oxidation with an inhibition rate over 85% at 1.0–2.0 mg/mL. The extract was effective in scavenging superoxide radical in a concentration dependent fashion with IC50 value of 1.03 mg/mL whereas that in scavenging abilities of hydroxyl radical was 0.66 mg/mL. 1,1-Diphenyl-2-picry...
Published on Mar 1, 2012in European Food Research and Technology2.06
Barbara Stachowiak1
Estimated H-index: 1
,
Julita Reguła1
Estimated H-index: 1
Edible macromycetes for ages have been used by humans, not only as a source of food, but medicinal resources as well; however, this happened first of all in the civilizations of the East. In recent years, they have become subjects of numerous scientific studies in Europe and United States. It was shown that they constitute a rich source of bioactive compounds exhibiting antitumor, hypocholesterolemic, immunosuppressive, antioxidant, antimicrobial and anti-inflammatory properties. These compounds...
Published on Feb 1, 2012in Carbohydrate Polymers6.04
Cunshan Zhou14
Estimated H-index: 14
(Jiangsu University),
Xiaojie Yu2
Estimated H-index: 2
(Zhejiang A & F University)
+ 2 AuthorsHaile Ma24
Estimated H-index: 24
(Jiangsu University)
Abstract The chemical structure and antioxidant of natural and ultrasonic degraded polysaccharides from Porphyra yezoensis Udea was investigated. The degraded polysaccharide (PYPS UD ) was purified, and F2 (a homogeneous fraction) was obtained. FT-IR, 1 H and 13 C NMR spectral analysis revealed that F2 have typical porphyran structure. It has a backbone of alternating (1 → 4)-3,6-anhydro-α- l -galactopyranose) units and (1 → 3)-linked β- d -galactose or (1 → 4)-linked α- l -galactose 6-sulfate u...
Published on Aug 1, 2011in Carbohydrate Polymers6.04
Tiffany Chien-Ting Lo4
Estimated H-index: 4
(NCTU: National Chiao Tung University),
Cheng Allen Chang12
Estimated H-index: 12
(NYMU: National Yang-Ming University)
+ 2 AuthorsJen-Fon Jen26
Estimated H-index: 26
(NCHU: National Chung Hsing University)
Abstract The relationships of antioxidant properties (AOPs), measured by four conventional in vitro methods, with monosaccharides and glycosyl linkages in the polysaccharide, were evaluated using multiple linear regression analysis with minor modifications. Polysaccharides extracted from culture broth filtrates of Lentinula edodes were used as model samples for evaluation. Results indicate that the composition of monosaccharides and the type of glycosyl linkage modulates the AOPs of the polysacc...
Published on May 1, 2011in Food Chemistry5.40
Josiana A. Vaz8
Estimated H-index: 8
,
Lillian Barros46
Estimated H-index: 46
(IPN: Instituto Politécnico Nacional)
+ 3 AuthorsIsabel C.F.R. Ferreira61
Estimated H-index: 61
(IPN: Instituto Politécnico Nacional)
Abstract Mushrooms have become attractive as functional foods and as a source of physiologically beneficial bioactive compounds. Herein, we describe and compare the chemical constituents (phenolic compounds, macronutrients, sugars, fatty acids, tocopherols and ascorbic acid) of four wild edible mushrooms widely appreciated in gastronomy: Armillaria mellea (Vahl) P. Kumm., Calocybe gambosa (Fr.) Donk, Clitocybe odora (Fr.) P. Kumm., Coprinus comatus (O.F. Mull.) Pers. Furthermore, the antioxidant...
Published on Jan 1, 2011in African Journal of Biotechnology
Ming-Yeou Lung7
Estimated H-index: 7
(MUST: Minghsin University of Science and Technology),
Yu-Cheng Chang2
Estimated H-index: 2
Antioxidant properties of exopolysaccharides (EPS) and intracellular polysaccharides (IPS) obtained respectively from mycelia and filtrates of submerged culture by Armillaria mellea in a 20-L stirred tank bioreactor were investigated. Effective production of EPS and IPS is available by submerged culture of A. mellea with respective number average molecular weights and protein/polysaccharide ratios as 7.68×10 6 Da and 7.68% as well as 5.65×10 6 Da and 5.26%. Both EPS and IPS exhibit powerful anti...
Cited By33
Newest
Published on Oct 1, 2019in Acta Physiologiae Plantarum1.61
Xi Yang3
Estimated H-index: 3
(University of Tsukuba),
Jingtong Zhang (University of Tsukuba)+ 4 AuthorsZhenya Zhang32
Estimated H-index: 32
(University of Tsukuba)
In this study, adventitious roots of Helicteres angustifolia L. (H. angustifolia) were generated on Murashige and Skoog salt mixture (MS) medium with auxin and ascorbic acid as additives. The root initiation ratio reached 100% on MS medium supplemented with 3.0 mg/L NAA and 0.4 mg/L ascorbic acid at 28 °C. The root number reached 15 ± 2 per leaf explant with a length of 1.70 ± 0.52 cm. Moreover, the cultured roots exhibited similar functional groups with wild plant roots and produced abundant cl...
Published on Sep 1, 2019in Food Research International3.58
Francisco Campos Junior1
Estimated H-index: 1
(State University of Campinas),
Mateus Henrique Petrarca3
Estimated H-index: 3
(State University of Campinas)
+ 2 AuthorsHelena Teixeira Godoy27
Estimated H-index: 27
(State University of Campinas)
Abstract A “green” analytical method is reported for the determination of phenolic acids in mushrooms. The sample preparation involves aqueous extraction based on acid hydrolysis, followed by analysis of extracts by capillary electrophoresis with diode array detector (CE-DAD). A central composite design was used to obtain the optimum conditions for the extraction of compounds from mushrooms, including the concentration of hydrochloric acid (2 mol·L−1), temperature (80 °C) and time (30 min). The ...
Published on Jul 1, 2019in International Journal of Biological Macromolecules4.78
Yaqin Xu6
Estimated H-index: 6
(NEAU: Northeast Agricultural University),
Ningyue Liu1
Estimated H-index: 1
(NEAU: Northeast Agricultural University)
+ 5 AuthorsLibo Wang5
Estimated H-index: 5
(NEAU: Northeast Agricultural University)
Abstract A native polysaccharide (RCPI) with sugar content of 79.94 ± 0.57% was isolated from raspberry fruits. A degraded polysaccharide (DRCPI) with sugar content of 83.82 ± 0.71% was obtained from the crude polysaccharides after degradation by Fe 2+ -H 2 O 2 treatment. The molecular weights of RCPI and DRCPI were determined to be 411,000 and 8010 Da, respectively. GC analysis revealed that both RCPI and DRCPI were composed of the same monosaccharides (rhamnose, arabinose, xylose, mannose, glu...
Published on May 1, 2019in International Journal of Biological Macromolecules4.78
Tayebeh Fooladi1
Estimated H-index: 1
(Alzahra University),
Mohammad Reza Soudi13
Estimated H-index: 13
(Alzahra University)
+ 2 AuthorsMajid M. Heravi8
Estimated H-index: 8
(Alzahra University)
Abstract Fungal exopolysaccharides are powerful resources of medicinal applications. Neopestalotiopsis sp. SKE15 was isolated and identified according to phenotypical and genotypical analyses (GenBank Accession No. MG649986). The exopolysaccharide (EPS) was produced by cultivation of mycelia in broth culture and extracted. The production was optimized to 2.02 g/l after selection of agitation, temperature, FeSO 4 and K 2 HPO 4 concentrations as the most influencing factors using Placket-Burman de...
Published on Apr 1, 2019in Industrial Crops and Products4.19
Yuntao Liu (Sichuan Agricultural University), Yixi Liu1
Estimated H-index: 1
(Sichuan Agricultural University)
+ 9 AuthorsPengfei Yin (Sichuan Agricultural University)
Abstract Catathelasma ventricosum is a popular edible officinal mushroom in southwest China. The mycelial polysaccharides from Catathelasma ventricosum (mCVPS) mainly composed of β -glucan which was widely regarded as a kind of prebiotic. In order to improve its yield, a mutant strain (mSM-105) by UV mutagenesis combined with ethyl methane sulfonate (EMS) treatment was developed, which showed a higher β -glucan production than the parent strain (SM-16). Results showed that mSM-105 possessed the ...
Published on Mar 1, 2019in South African Journal of Botany1.50
Xi Yang3
Estimated H-index: 3
(University of Tsukuba),
Zhongfang Lei23
Estimated H-index: 23
(University of Tsukuba)
+ 3 AuthorsZhenya Zhang32
Estimated H-index: 32
(University of Tsukuba)
Abstract Helicteres angustifolia L. ( H.angustifolia ) is an important medicinal plant. This study investigated the phytochemical characteristics of callus suspension cultures of H.angustifolia and its bioactive functions, such as antioxidant, antidiabetic and immunomodulatory activities. Results demonstrated that the cultures were rich in phenolic compounds, flavonoids, terpenoids, saponins and triterpenoids. Compared to the equivalent wild roots, the cultures yielded larger amounts of total ph...
Published on Feb 1, 2019in Carbohydrate Polymers6.04
Yan Lu1
Estimated H-index: 1
(SDU: Shandong University),
Lei Xu1
Estimated H-index: 1
(SDU: Shandong University)
+ 5 AuthorsKaoshan Chen10
Estimated H-index: 10
(Wannan Medical College)
Abstract A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis . TPS had a weight-average molecular mass of 3.074 × 10 5 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%). The major backbone of TPS was →6-α- d -Galp-1→5-β- d -Manf-1→5,6-β- d -Manf-1→5,6-β- d -Manf-1→, and the side chains are α- d -Glcp-1→4-α- d -Glcp-1→, β- d -Galf-1→, and α- d -Glcp-1→. In addition, we...
Published on Feb 1, 2019in International Journal of Biological Macromolecules4.78
Enwei Wei1
Estimated H-index: 1
(JLU: Jilin University),
Rui Yang1
Estimated H-index: 1
+ 5 AuthorsHongli Zhou2
Estimated H-index: 2
Abstract Seabuckthorn berries are rich in various bioactive components and used as a traditional medicine for a long time. Until now, little information is available for the extraction of polysaccharides from seabuckthorn berries (PSB) by linking antioxidant activity and microwave power. In this study, microwave-assisted extraction, characterization, in vitro and in vivo antioxidant activities of PSB were explored. The maximum PSB extraction yield of 0.264 ± 0.005% was obtained under the optimal...
Published on Dec 13, 2018in International Journal of Molecular Sciences4.18
Na Xu2
Estimated H-index: 2
,
Yi Lu + 2 AuthorsYonghai Sun3
Estimated H-index: 3
Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress i...
Published on Dec 1, 2018in Ultrasonics Sonochemistry7.28
Yaqin Xu6
Estimated H-index: 6
(NEAU: Northeast Agricultural University),
Yingying Guo1
Estimated H-index: 1
(NEAU: Northeast Agricultural University)
+ 5 AuthorsYu Yang6
Estimated H-index: 6
(NEAU: Northeast Agricultural University)
Abstract In this study, the influence of ultrasound irradiation on the characterization and bioactivities of the polysaccharide from blackcurrant fruits (BCP, molecular weight: M w = 3.26 × 10 4 kDa) was investigated. Two degraded polysaccharides (U-400, M w = 1.89 × 10 4 kDa, and U-600, M w = 1.32 × 10 4 kDa) were obtained by different ultrasound powers of 400 W and 600 W, respectively. Compared with BCP, U-400 and U-600 showed 63.52% and 68.85% reductions in the particle size ( Z avg ), respec...
View next paperStructural analysis and antioxidant activities of polysaccharide isolated from Jinqian mushroom.