Extension of donor lung preservation with hypothermic storage after normothermic ex vivo lung perfusion

Ex vivo lung perfusion (EVLP) allows normothermic evaluation and treatment of donor lungs not currently acceptable for transplant and improves organ use. Donor lungs undergo a period of cold preservation before (cold ischemic time [CIT]-1) and after (CIT-2) EVLP. We investigated the effect of an extended CIT-2 on lung function after transplantation.Explanted pig lungs, preserved in low-potassium dextran flush (Perfadex) at 4°C for 10 hours, were...