Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

Short‐chain fatty acids (SCFAs) are the main metabolites of the intestinal flora and play an important role in the interaction between the intestinal flora and host metabolism. Therefore, reliable methods are needed to accurately measure SCFAs concentrations. SCFAs are commonly analyzed by gas chromatography–mass spectrometry (GC‐MS), which requires lengthy sample treatments and a long run time. This study aimed to develop a fast GC method with...