Original paper

Continuous evolution of SpCas9 variants compatible with non-G PAMs

Volume: 38, Issue: 4, Pages: 471 - 481
Published: Feb 10, 2020
Abstract
The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using phage-assisted non-continuous evolution, three new phage-assisted continuous evolution strategies for DNA binding and a secondary...
Paper Details
Title
Continuous evolution of SpCas9 variants compatible with non-G PAMs
Published Date
Feb 10, 2020
Volume
38
Issue
4
Pages
471 - 481
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