Sperm proteomic changes associated with early embryo quality after ICSI

Research question Do alterations of human sperm protein profile affect embryo quality? Design Sperm proteins from 27 infertile couples undergoing intracytoplasmic sperm injection (ICSI) were extracted and digested. The resulting peptides were labelled using tandem mass tags, separated by two-dimensional liquid chromatography, and identified and quantified using tandem mass spectrometry. Subsequently, sperm protein and peptide abundance were...