Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues
Abstract
Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation and decomplexation of the processive cellulase Cel7A and its insoluble substrate, cellulose. The method enabled detailed kinetic and thermodynamic analyses of ligand binding in a heterogeneous...
Paper Details
Title
Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues
Published Date
Feb 1, 2020
Volume
295
Issue
6
Pages
1454 - 1463
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