Stir membrane liquid phase microextraction of tetracyclines using switchable hydrophilicity solvents followed by high-performance liquid chromatography

A novel approach for stir membrane liquid phase microextraction of tetracyclines from biological fluids was developed. The microextraction procedure assumed in situ formation of microdroplets of a hydrophobic medium-chain fatty acid (extraction solvent) from homogeneous sample solution containing water-soluble medium-chain fatty acid salt by acidification and simultaneous analytes extraction and organic phase separation into pores of stir...