CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

The S-layer is a proteinaceous surface lattice found in the cell envelope of bacteria and archaea. In most archaea, a glycosylated S-layer constitutes the sole cell wall and there is evidence that it contributes to cell shape maintenance and stress resilience. Here we use a gene-knockdown technology based on an endogenous CRISPR type III complex to gradually silence slaB , which encodes the S-layer membrane anchor in the hyperthermophilic...