Original paper
Local redox conditions in cells imaged via non-fluorescent transient states of NAD(P)H
Abstract
The autofluorescent coenzyme nicotinamide adenine dinucleotide (NADH) and its phosphorylated form (NADPH) are major determinants of cellular redox balance. Both their fluorescence intensities and lifetimes are extensively used as label-free readouts in cellular metabolic imaging studies. Here, we introduce fluorescence blinking of NAD(P)H, as an additional, orthogonal readout in such studies. Blinking of fluorophores and their underlying dark...
Paper Details
Title
Local redox conditions in cells imaged via non-fluorescent transient states of NAD(P)H
Published Date
Oct 21, 2019
Journal
Volume
9
Issue
1
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Notes
History