Effects of the 2-Substituted Adenosine-1,3-diazaphenoxazine 5′-Triphosphate Derivatives on the Single Nucleotide Primer Extension Reaction by DNA Polymerase

The adenosine triphosphate derivatives of 2-oxo-1,3-diazaphenoxazine (dAdapTP) showed a significant discrimination ability for the template strand including that between 8-oxo-2′-deoxyguanosine (8-oxodG) and 2′-deoxyguanosine (dG) by the single nucleotide primer extension reaction using the Klenow Fragment. In this study, we synthesized new dAdapTP derivatives, i.e., 2-amino-dAdapTP, 2-chloro-dAdapTP and 2-iodo-dAdapTP, to investigate the effect...