Original paper
Efficient Tandem LysC/Trypsin Digestion in Detergent Conditions
Abstract
All shotgun proteomics experiments rely on efficient proteolysis steps for sensitive peptide/protein identification and quantification. Previous reports suggest that the sequential tandem LysC/trypsin digest yields higher recovery of fully tryptic peptides than single-tryptic proteolysis. Based on the previous studies, it is assumed that the advantageous effect of tandem proteolysis requires a high sample denaturation state for the initial LysC...
Paper Details
Title
Efficient Tandem LysC/Trypsin Digestion in Detergent Conditions
Published Date
Sep 30, 2019
Journal
Volume
19
Issue
20
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Notes
History