Two-step FRET as a tool for probing the amyloid state of proteins

Amyloid fibrils, a special class of protein aggregates with a core β-sheet structure, are currently associated with a number of human disorders. The fluorescence-based techniques play essential role in detection and structural characterization of amyloid assemblies. The amyloid-sensing potential of the two-step Förster resonance energy transfer has been assessed using the three-chromophore system containing a benzothiazole dye Thioflavin T, a...