Diet‐induced microbial autofluorescence confounds flow cytometry of ex vivo isolated fecal microbes
Abstract
Microbial flow cytometry is a powerful emerging technology with a broad range of applications including the study of complex microbial communities. Immunologists are increasingly using this technology to study antibody responses against pathogenic and commensal microbes. We employed microbial flow cytometry to quantify the proportion of fecal microbes bound by six different Ig isotypes: IgA, IgM, IgG1, IgG2b, IgG2c, and IgG3. In healthy mammals,...
Paper Details
Title
Diet‐induced microbial autofluorescence confounds flow cytometry of ex vivo isolated fecal microbes
Published Date
Sep 13, 2019
Volume
49
Issue
12
Pages
2252 - 2254
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