Validation of a Biochip Chemiluminescent Immunoassay for Multi-Mycotoxins Screening in Maize (Zea mays L.)

A biochip chemiluminescent immunoassay was validated for multi-mycotoxins screening in maize. Screened mycotoxins were aflatoxins B1 (AFB1) and G1 (AFG1) ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2), fumonisins (sum of FB1 and FB2) and deoxynivalenol (DON). The method included a single extraction step with acetonitrile:methanol:water (50:40:10, v/v/v). Spiked samples were fortified at 250 μg/kg for FB1 + FB2, 1 μg/kg for AFB1 and AFG1,...