Pef-assisted Supercritical CO2 Extraction of Pigments from Microalgae Nannochloropsis Oceanica in a Continuous Flow System

This study aimed at evaluating the effect of pulsed electric fields (PEF) pre-treatment on the extractability of pigments (total carotenes, chlorophyll a) from microalgae Nannochloropsis oceanica by supercritical CO2 (scCO2) extraction. Aqueous microalgal suspensions (1% w/w) added with 10% ethanol were treated in a continuous flow unit with either PEF at different field strengths (10–30 kV/cm) and total specific energy inputs (20–100 kJ/kg), or...