Solvent and temperature effects of accelerated solvent extraction (ASE) with Ultra-high pressure liquid chromatography (UHPLC-PDA) technique for determination of Piperine and its ICP-MS analysis

Black pepper (Piper nigrum), known as “king of spices” is the most widely used spice in the world. Though a number of marketed food grade products are available for black pepper, however, the labels does not mention the quality of active drug Piperine (PPN) and level of toxic elements. The study aims to develop a rapid, effective and reliable method for extraction of PPN and a short and sensitive method for quantification of PPN in commercial...