Multiplex PCR targeting lineage‐specific SNPs: A highly efficient and simple approach to block out predator sequences in molecular gut content analysis

Volume: 10, Issue: 7, Pages: 982 - 993
Published: May 2, 2019
Abstract
Food webs form the basis of biological communities, though empirical research has been hindered by difficulties in quantifying interactions. Metabarcoding from predator gut content extractions with universal primers promises to provide simple and rapid insights into food web interactions. However, the highly overabundant predator DNA often completely out‐competes that of the digested prey DNA during PCR , impeding the ability to assess the...
Paper Details
Title
Multiplex PCR targeting lineage‐specific SNPs: A highly efficient and simple approach to block out predator sequences in molecular gut content analysis
Published Date
May 2, 2019
Volume
10
Issue
7
Pages
982 - 993
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