Efficient induction of heritable inversions in plant genomes using theCRISPR/Cas system

Summary During the evolution of plant genomes, sequence inversions occurred repeatedly making the respective regions inaccessible for meiotic recombination and thus for breeding. Therefore, it is important to develop technologies that allow the induction of inversions within chromosomes in a directed and efficient manner. Using the Cas9 nuclease from Staphylococcus aureus ( Sa Cas9), we were able to obtain scarless heritable inversions with high...