SAA-Cas9: A tunable genome editing system with increased bio-safety and reduced off-target effects

Silks are natural protein biopolymers with desirable mechanical properties and play crucial roles in insect survival and reproduction. However, the mechanisms by which large amounts of silk fibroin are efficiently secreted from the protein production organs (silk glands) remain elusive. Here, we focus on a dominant silkworm mutation, naked pupa (Nd), which enables carriers to lose spinning behaviors, produce a deficiency of silk fibroin...