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Sanyuan Ma
Southwest University
GeneGenome editingBombyx moriGeneticsBiology
53Publications
14H-index
883Citations
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Publications 46
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#1Jiasong Chang (SWU: Southwest University)H-Index: 7
#2Xiaoxu Chen (SWU: Southwest University)H-Index: 2
Last. Qingyou Xia (SWU: Southwest University)H-Index: 44
view all 9 authors...
Abstract The CRISPR/Cas (clustered regularly interspaced short palindromic repeat technology/CRISPR-associated protein) is a widely used and powerful research tool in biosciences and a promising therapeutic agent for treating genetic diseases. Mutations induced by Cas9 are generally considered stochastic and unpredictable, thus hindering its applications where precise genetic alternations are required. Here, through deep sequencing and analysis of genome editing outcomes of multiple sites in fou...
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#1Jiasong Chang (SWU: Southwest University)H-Index: 7
#2Ruolin Wang (SWU: Southwest University)H-Index: 1
Last. Sanyuan Ma (SWU: Southwest University)H-Index: 14
view all 10 authors...
: High-throughput genetic screens are powerful methods to interrogate gene function on a genome-wide scale and identify genes responsible to certain stresses. Here, we developed a piggyBac strategy to deliver pooled sgRNA libraries stably into cell lines. We used this strategy to conduct a screen based on genome wide clustered regularly interspaced short palindromic repeat technology (CRISPR)-Cas9 in Bombyx mori cells. We first constructed a single guide RNA (sgRNA) library containing 94,000 sgR...
1 CitationsSource
#1Yue Liu (SWU: Southwest University)H-Index: 4
#2Yufeng Li (SWU: Southwest University)H-Index: 1
view all 7 authors...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR) system and the Cas9-derived proteins have been applied to genome editing in various organisms. Traditional Cas9 is typically used to knockout genes or specific DNA fragments based on the generation of double-stranded breaks, whereas nCas9 and dCas9 are fused with effectors to perform base pair transitions or epigenetic modification and regulation. However, this system has off-target effects and can cause genomic stru...
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#1Jiasong Chang (SWU: Southwest University)H-Index: 7
#2Xiaoxu Chen (SWU: Southwest University)H-Index: 2
Last. Qingyou Xia (SWU: Southwest University)H-Index: 44
view all 8 authors...
As a primary strategy for production of biological drugs, recombinant proteins produced by transient transfection of mammalian cells are essential for both basic research and industrial production. Here, we established a high-throughput screening platform for improving the expression levels of recombinant proteins. In total, 10,011 small molecule compounds were screened through our platform. After two rounds of screening, we identified two compounds, Apicidin and M-344, that significantly enhanc...
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#1Xiaogang Wang (SWU: Southwest University)H-Index: 8
#2Sanyuan Ma (SWU: Southwest University)H-Index: 14
Last. QingyouXia (SWU: Southwest University)
view all 7 authors...
: The recently developed clustered regularly interspaced short palindromic repeats (CRISPR)-based techniques have made it possible to reprogram target gene expression without cloning complementary DNA or disturbing genomic sequence in mammalian cells and several multicellular organisms. We previously showed that CRISPR-associated protein 9 (Cas9) and CRISPR from Prevotella and Francisella 1 (Cpf1) could induce target mutations, deletions, inversions, and duplications both singly and multiplex in...
4 CitationsSource
#1Sanyuan Ma (SWU: Southwest University)H-Index: 14
#1Sanyuan Ma (SWU: Southwest University)H-Index: 1
Last. QingyouXia (SWU: Southwest University)
view all 3 authors...
In recent years, research in life sciences has been remarkably revolutionized owing to the establishment, development and application of genome editing technologies. Genome editing has not only accelerated fundamental research but has also shown promising applications in agricultural breeding and therapy. In particular, the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology has become an indispensable tool in molecular biology owing to its high efficacy and simplicity...
6 CitationsSource
#1Sanyuan Ma (SWU: Southwest University)H-Index: 14
#2Yuanyuan Liu (SWU: Southwest University)H-Index: 8
Last. QingyouXia (SWU: Southwest University)
view all 7 authors...
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#1Ling Jia (SWU: Southwest University)H-Index: 1
#2Sanyuan Ma (SWU: Southwest University)H-Index: 14
Last. QingyouXia (SWU: Southwest University)
view all 8 authors...
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#1Wei‐Qing Xing (SWU: Southwest University)H-Index: 1
#2Sanyuan Ma (SWU: Southwest University)H-Index: 14
Last. QingyouXia (SWU: Southwest University)
view all 4 authors...
1 CitationsSource
#1Zhaoming Dong (SWU: Southwest University)H-Index: 12
#2Kaiyu Guo (SWU: Southwest University)H-Index: 3
Last. Ping Zhao (SWU: Southwest University)H-Index: 28
view all 11 authors...
Abstract Sericins are large proteins with molecular weights >70 kDa. Three sericin genes were reported in the silkworm, including sericin 1 , sericin 2 and sericin 3 . In this study, we have identified a new sericin gene and designated it as sericin 4 . The sequence, exon-intron structure, alternative splicing, and translation products of this gene have been described in this study. Quantitative RT-PCR analysis indicates that sericin 4 is expressed in the middle silk gland. Immunofluorescence re...
4 CitationsSource
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