Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs
Abstract
Target-specific genome editing using engineered nucleases has become widespread in various fields. Long gene knock-in and single-base substitutions can be performed by homologous recombination (HR), but the efficiency is usually very low. To improve the efficiency of knock-in with single-stranded oligo DNA nucleotides (ssODNs), we have investigated optimal design of ssODNs in terms of the blocking mutation, orientation, size, and length of...
Paper Details
Title
Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs
Published Date
Mar 18, 2019
Journal
Volume
9
Issue
1
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